To establish the quality standard of Chuanbeizhike mixture.Methods:Thin layer chromatography (TLC) was used to identify Fructus Aurantii, Radix Platycodonis in Chuanbeizhike Mixture. Naringin in Fructus Aurantii was determined by high performance liquid chromatography (HPLC).Results:The TLC spots of Fructus Aurantii and Radix Platycodonis were clear with good resolution and free of interference of negative samples. The calibration curve of HPLC was linear in the range of 160～800ng (r=0.999 9).The average recovery rate was 98.90%, RSD=1.32%.Conclusion:The method is rapid, curate, sensitive and reliable, and it can be used to control the quality of Chuanbeizhike Mixture.