| 引用本文:邹海艳 刘珊珊 李 佳 马 晗 赵 晖.竹节参皂苷类化合物HPLC指纹图谱研究[J].世界中医药,2014,9(10):. |
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| 竹节参皂苷类化合物HPLC指纹图谱研究 |
| Studies on the HPLC Fingerprints of Saponins in Rhizoma Panax Japonicus |
| 投稿时间：2014-04-14 |
| DOI：10.3969/j.issn.1673-7202.2014.10.033 |
| 中文关键词: 竹节参 皂苷类化合物 HPLC 指纹图谱 |
| English Keywords:Rhizoma Panax Japonicus Saponins HPLC Fingerprint |
| 基金项目:国家自然科学基金（编号：81403095）；北京市属高校青年拔尖人才培育项目（编号：CIT&TCD201404175，CIT&TCD201404184）；首都中医药护理专项（编号：12ZYH08） |
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| 中文摘要: |
| 目的：建立竹节参药材皂苷类成分HPLC指纹图谱的研究方法，为竹节参药材的质量控制提供依据。方法：采用Hibar C18（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-0.1％的磷酸水溶液为流动相，梯度洗脱，流速1.0 mL·min-1，检测波长203 nm，柱温30 ℃；测定了7批市售竹节参药材的HPLC指纹图谱。结果：竹节参的指纹图谱共检出12个共有峰，通过对照品比对指认了其中的7个共有皂苷类成分，7批药材中有6批相似度均达到0.9以上。结论：该方法精密度、稳定性、重复性好，特征性及专属性强，可用于竹节参药材的质量控制。 |
| English Summary: |
| To establish HPLC fingerprint method for quality control of saponins in Rhizoma Panax Japonicus.Methods:The HPLC method was conducted based on a Hibar C18(4.6 mm×250 mm,5 μm) column,with acetonitrile-0.1% orthophosphoric acid as mobile phase by gradient elution.The detection wavelength was at 203 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 30 ℃.Seven samples from different sources were analyzed by HPLC.Results:Twelve main marker peaks were selected in the standard fingerprint and 7 peaks of saponins were identified.The similarity of 6 samples was over 0.9.Conclusion:The precision,repeatability and stability of the method were up to standard，and this method can be used for quality evaluation of Rhizoma Panax Japonicus. |
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