世界中医药

目的：初步探索醒脾解郁方对抑郁模型大鼠的抗抑郁行为和血清激素、神经递质变化的影响。方法：本实验将SD大鼠随机分为4组：正常组、模型对照组、舍曲林对照组、中药（石菖蒲、西洋参、郁金、熟地黄）组，采用21 d慢性不可预见性温和应激建立抑郁大鼠模型，以糖水偏爱和旷野试验移动距离评价各组大鼠抑郁行为，用ELISA法测定大鼠血清5-羟色胺、皮质酮含量，并比较醒脾解郁方对其影响的作用。结果：21 d慢性应激建立大鼠抑郁模型后，与正常组比较，模型组体重降低明显（P<0.01），糖水偏爱率、旷场试验移动距离显著下降（P<0.01），同时模型组血清皮质酮含量显著增加（P<0.01）、5-HT明显降低（P<0.01）；与模型组比较，中药组和舍曲林组均能显著改善大鼠抑郁行为（P<0.05），并且醒脾解郁方和舍曲林均对抑郁大鼠血清皮质酮、5-HT均有显著逆转作用（P<0.05）。结论：醒脾解郁方具有抗抑郁作用，并与舍曲林效果相当，并能逆转抑郁模型大鼠血清皮质酮、5-HT的作用，这可能是其改善抑郁的作用机制。

English Summary:

To preliminarily explore the effect of Xingpijieyu decoction on depressive behavior and serum 5-HT and corticosterone on depression model rats.Methods:SD rats were randomly divided into 4 groups:normal group，model control group，sertraline control group，and traditional Chinese medicine (stone calamus，American ginseng，radix curcumae，cultivated land) group.21 days chronic unpredictable mild stress was adopted to establish the rat model of depression，using sucrose preference and open-field test to evaluate depression of rats，and compare the change of 5-HT and corticosterone form every groups of rats by ELISA.Results:After 21 days of chronic stress depression rats model made，compared with normal group，weight，preference for sugar water rate and open-field total distance of the model group were significantly decreased (P<0.01)，and serum corticosterone increased significantly (P<0.01)，serum 5-HT significantly decreased (P<0.01); Compared with model group，sertraline group and Chinese medicine group both could improve depressive behavior (P<0.05)，and reverse serum 5-HT and corticosterone significantly (P<0.05).Conclusion:Xingpijieyu Decoction can improve chronic unpredictable stress depression rats model depression function，which is the same as sertraline controls.Xingpijieyu decoction also could reverse erum 5-HT and corticosterone from depression rats，which might be related to its improvement of depression.

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