| 引用本文:侯 丽1 许 晶2 王 婧1 郭晓青1 宋延平3.新加良附方联合5-Fu对荷人胃癌裸鼠血清VEGF、bFGF水平的影响[J].世界中医药,2014,9(12):. |
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| 新加良附方联合5-Fu对荷人胃癌裸鼠血清VEGF、bFGF水平的影响 |
| The Effects of Modified Liang-Fu Formula Combined with 5-Fu on VEGF，bFGF Expression of Human Gastric Cancer Xenografts in Nude Mice |
| 投稿时间：2014-02-11 |
| DOI：10.3969/j.issn.1673-7202.2014.12.020 |
| 中文关键词: 新加良附方 新加良附方联合5-Fu VEGF bFGF |
| English Keywords:Modified Liang-fu formula Modified Liang-fu formula combined with 5-Fu VEGF bFGF |
| 基金项目:北京中医药大学科学科研基地建设项目北京市重点实验室（中医内科学）——新加良附方抗胃癌效应机制研究（编号：2011-JDJS-02） |
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| 中文摘要: |
| 目的：观察新加良附方联合5-Fu对荷人胃癌移植瘤裸鼠血清血管内皮生长因子（Vascular Endothelial Growth Factor，VEGF）、碱性成纤维生长因子(Basic Fibroblast Growth Factor，bFGF)水平的影响。方法：建立移植性人胃癌细胞（SGC-7901）裸鼠移植瘤模型，并将模型动物随机分为空白组、5-Fu组及新加良附方高、中、低剂量组、联合给药组（新加良附中剂量+5-Fu组）6组。新加良附方高、中、低剂量组给药剂量分别为10 g·d-1·kg-1、5 g·d-1·kg-1、2.5 g·d-1·kg-1；5-Fu组给药剂量为17 mg·d-1·kg-1；空白组给予等量无菌生理盐水，新加良附方联合5-Fu组剂量为新加良附方中剂量（5 g·d-1·kg-1）联合应用5-Fu（17 mg·d-1·kg-1）。给药结束后，采用免疫学方法(ELISA)检测裸鼠血清中VEGF、bFGF的含量。结果：VEGF含量显示：联合给药组，5-Fu组，新加良附大、中、小剂量组裸鼠血清中含量较空白对照组均明显降低(P<0.01)，新加良附各剂量组对VEGF含量减低作用具有一定的剂量依赖性，联合用药组VEGF含量低于5-Fu组、新加良附中剂量组，但无统计学意义（P>0.05）。bFGF含量显示：联合给药组，5-Fu组，新加良附大、中、小剂量组裸鼠血清中含量较空白对照组均明显降低(P<0.01)。结论：新加良附方可以下调人胃癌裸鼠的血清中VEGF、bFGF表达水平，提示新加良附方具有一定抗胃癌新生血管生成作用。 |
| English Summary: |
| To observe the effect of Modified Liang-fu Formula (MLFF) combined with 5-Fu on VEGF and bFGF expression in the serum of human gastric cancer xenografted nude mice.Methods:The animal model of SGC-7901 xenografted nude mice was established，and all the mice were randomly divided into 6 groups; namely model control group (the same amount of normal sterile saline)，5-Fu group (17 mg·d-1·kg-1)，MLFF high-dose group (10 g·d-1·kg-1)，MLFF medium-dose group (5 g·d-1·kg-1)，MLFF low-dose group (2.5 g·d-1·kg-1) and drug combination group (5 g·d-1·kg-1 MLFF combined with 17 mg·d-1·kg-1 5-Fu).After 10 days of continuous treatment，eyeball blood was withdrew，the mice were killed，and the expression of VEGF and bFGF in the serum were detected using ELISA method.Results:Compared to model control group，the expression levels of VEGF in nude mice serum were significantly reduced in 5-Fu group，MLFF high-dose group，MLFF medium-dose group，MLFF low-dose group and drug combination group，respectively (P<0.01)，while MLFF reduced VEGF expression in a dose-dependent manner.The combination group showed a lower level of VEGF compared to 5-Fu group and MLFF medium-dose group，but there was no significant difference (P<0.05).Meanwhile，compared to model control group，the expression level of bFGF in nude mice serum was significantly decreased in 5-Fu group，MLFF high-dose group，MLFF medium-dose group，MLFF low-dose group and drug combination group，respectively (P<0.01).Conclusion:MLFF can down-regulate the expression of VEGF，bFGF in the serum of human gastric cancer xenografted nude mice.It indicates that MLFF has the potential to serve as anti-angiogenesis agent for gastric cancer. |
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