养心通脉有效部位方对BMSCs移植心血瘀阻证心肌的实验研究

doi:10.3969/j.issn.1673-7202.2015.01.020

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养心通脉有效部位方对BMSCs移植心血瘀阻证心肌的实验研究
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                        10.3969/j.issn.1673-7202.2015.01.020
                    
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基金项目:国家自然科学基金项目（编号：81102595）；广西自然科学基金（编号：2012GXNSFAA053113，2013GXNSFAA019126）；广西科学研究与技术开发计划项目（编号：桂科合1347004-26）；广西壮族自治区中医药民族医药自筹经费科研课题（编号：GZZC1223）

Experimental Study on Effect of Active Principle Region of Yangxing Tongmai Formula on BMSCs Transplantation in Myocardium with Blood Stasis Syndrome

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目的：观察养心通脉部位方（Active Principle Region of Yangxing Tongmai Formula，apr-YTF）对大鼠骨髓间充质干细胞（Bone Mesemchymal Stem Cells，BMSCs）移植心血瘀阻证心肌后分化为心肌样细胞的作用。方法：采用“冠脉结扎法”结合血流变学改变建立大鼠心血瘀阻证模型。然后将大鼠分为(apr-YTF)+BMSCs组、集落刺激因子(rhG-CSF)+BMSCs组、NS+BMSCs组、NS＋DMEM组。原代培养大鼠BMSCs，体外溴氮胞苷（5-bromouracil Deoxyriboside Brdu）标记后注射至大鼠心肌，采用双染法观察心肌组织中心肌结蛋白Desmin-Brdu和肌球蛋白重链（Myosin Heavy Chain MHC）-Brdu的表达。结果：apr-YTF干预BMSCs移植心血瘀阻证心肌28 d后经心肌结蛋白Desmin-Brdu抗体染色后，NS+DMEM组梗死边缘未见双染表达，在NS+BMSCs组、(rhG-CSF)+BMSCs和(apr-YTF)+BMSCs组均可有猩红色Brdu抗体和棕色Desmin蛋白的同时表达，其中(rhG-CSF)+BMSCs和(apr-YTF)+BMSCs组阳性细胞计数高于NS+BMSCs组（P<0.05）；(rhG-CSF)+BMSCs组和(apr-YTF)+BMSCs组两者之间差异无统计学意义（P>0.05）。结论：apr-YTF能促使移植于心血瘀阻证心肌环境后BMSCs向心肌细胞的方向分化。

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To observe the effect of active principle region of site Yangxing Tongmai Formula（apr-YTF) improving the rat bone marrow mesenchymal stem cells (BMSCs) transplanted in myocardium with blood stasis syndrome differentiated into myocardial like cells.Methods:Coronary ligation combining blood rheology changes was used to set up rat blood stasis syndrome models.Then the rats were divided into (apr-YTF) + BMSCs group，recombinant human granulocyte colony-stimulating factor + BMSCs group，NS + BMSCs group，and NS + DMEM group.Primary cultured rat BMSCs，in vitro labeled by 5-romouracil deoxyriboside Brdu was injected into rat myocardium，using double staining myocardial tissue myocardial desmin Desmin-Brdu and myosin heavy chain (myosin heavy chain MHC)-Brdu expression.Results:After 28 days Desmin-Brdu myocardial desmin antibody staining，NS + DMEM group infarct border no double staining expressed in NS + BMSCs group，(rhG-CSF) + BMSCs and (apr-YTF) + BMSCs group can had scarlet and brown Desmin antibody Brdu simultaneous expression of proteins，including (rhG-CSF) + BMSCs and (apr-YTF) + BMSCs group-positive cell counts higher than NS + BMSCs group (P<0.05); (rhG-CSF) + BMSCs group and (apr-YTF) + BMSCs had no difference (P>0.05).Conclusion:Apr-YTF can promote BMSCs transplanted myocardial environmental blood stasis syndrome differentiation to cardiomyocytes .

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陈夏,郑景辉,李勇华,袁肇凯,孙贵香,简维雄,黄献平.养心通脉有效部位方对BMSCs移植心血瘀阻证心肌的实验研究[J].世界中医药,2015,10(01).

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收稿日期:2014-04-02
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在线发布日期: 2015-01-28
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