To investigate the Banzhilian extracts effects on Notch1 of rats with aflatoxin B1-induced hepatic carcinoma.Methods: Sixty Wistar rats were randomly divided into 4 groups: normal control group，hepatocarcinoma model group，extracts high dose group and low dose group.Hepatic carcinoma model were induced by aflatoxin B1.The control group and the model group were given distilled water lavagely，the extracts groups were given different concentrations of extracts lavagely，qd.for a month.All rats were killed in the 4th week，measure weight，counted number of nodules on the surface of liver.Immunohistochemistry were used to detect the expression of Notch1.Results: Compared with the control group，the rats weight in other groups dropped significantly (P<0.05)，while the nodule number and Notch1 levels increased significantly (P<0.05); Compared with the model group，the rats weight in the extracts of high dose group and low dose group were increased (P<0.05)，while the nodule number and Notch1 levels were reduced considerably(P<0.05).Conclusion: Banzhilian extract could inhibit aggravation of liver carcinoma，which is probably related to the down-regulating of liver Notch1.