To observe the effect of pine in respiratory syncytial virus infection in the expression of TLR3 lung tissue and TLR4 protein in mice，and explore the immune regulation mechanism of the treatment of respiratory syncytial virus infection.Methods: 60 ICR mice were randomly divided into 5 groups: normal control group，model group，the antiviral oral liquid group，ribavirin，and pine needles.After intranasal administration of respiratory syncytial virus，observe TLR3 lung tissue and the expression of TLR4 protein.Results: The antiviral oral liquid，ribavirin，pine needles can reduce the TLR3 and the expression of TLR4 protein of lung tissue in mice，compared with model group with significant difference (P<0.05).Conclusion: Pine needles can inhibit the respiratory syncytial virus infection in the lung tissue of mice TLR3 and the expression of TLR4 protein，and may be one of the mechanisms of immune regulation.