To establish a high performance liquid phase method to measure the content of chlorogenic acid of Flos Lonicerae in Ganmao granules，and to ensure that the results are accurate and reliable by systematic methodology.Methods:The chromatographic column:Agilent TC-C18 column (4.6 × 150 mm，5 μ m，Agilent，American)，a mobile phase of methanol:-0.5% phosphoric acid solution (15:85)，the detection wavelength was 327 nm.Results:The chlorogenic acid in 25ug mL-1-800ug·mL-1 linear relationship was good，the linear regression equation was Y = 27.88X+2.128，correlation coefficient was 0.999 8 (n = 6); low，high concentrations of RSD precision，values were 0.59%，0.58%，0.44%; repetitive RSD 0.26%; low，recoveries.The high concentration were respectively 99.24%，99.43%，99.75%; the test solution was placed at room temperature for 8 h，and RSD was 0.29%.Conclusion:This method for determination of the content of chlorogenic acid in Flos Lonicerae cold granule is accurate and reliable with simple operation and short time.It can be used for the determination of chlorogenic acid in Flos Lonicerae in Ganmao granules.