| 引用本文:张风1，陶艳艳1,2，陈高峰1,2.改良PAS染色法在急性肝损伤中的应用[J].世界中医药,2015,10(02):. |
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| 改良PAS染色法在急性肝损伤中的应用 |
| Application of improved PAS staining in acute liver injury |
| 投稿时间：2014-12-26 |
| DOI：10.3969/j.issn.1673-7202.2015.02.007 |
| 中文关键词: 肝糖原 急性肝损伤 PAS染色法 改良 |
| English Keywords:Hepatic glycogen Acute hepatic injury Periodic acid-schiff (PAS) Improvement |
| 基金项目:国家自然科学基金项目(编号：81102701)；上海中医药大学实验技术人才队伍建设资助计划［编号：上中医（2012）人字18号］；上海市中医临床重点实验室(编号：14DZ2273200) |
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| 中文摘要: |
| 目的：观察改良肝脏糖原PAS染色法，并观察肝脏糖原染色在急性肝损伤中的应用。方法：复制CCl4急性肝损伤模型，首次100% CCl4 3 mL/kg皮下注射，此后50% CCl4橄榄油溶液2 mL/kg每周2次共4次皮下注射，诱导大鼠急性肝损伤模型。计算大鼠肝体比；HE染色观察肝组织炎症病理；试剂盒检测血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、总胆红素（TBil）、白蛋白（Alb）。肝脏常规PAS染色与改良PAS染色观察肝糖原染色。结果：与正常组相比，模型组ALT、AST活性与TBil含量明显升高（P<0.05），Alb含量明显降低（P<0.05）；HE染色示，模型组肝小叶结构排列紊乱，肝细胞脂肪变、气球样变明显。常规PAS染色，正常组肝组织PAS染色阳性占肝脏面积为32.38%±5.50%；与正常组相比，模型组肝组织PAS阳性染色明显减少(P<0.01)，占肝脏面积为8.60%±3.34%。改良PAS染色提示，正常组肝脏可见大量PAS阳性染色，占肝脏面积为75.50%±9.02%；与正常组相比，模型组肝组织PAS阳性染色明显减少(P<0.01)，占肝脏面积为17.61%±3.53%。在空白对照组与模型肝组织中，肝糖原改良PAS染色阳性率明显高于常规PAS染色法(P<0.01)。改良PAS染色肝糖原阳性染色面积更真实反映急性肝损伤程度。结论：改良肝脏糖原PAS染色法有助于急性肝损伤程度评估。 |
| English Summary: |
| To study in situ staining of glycogen by modified Periodic acid-schiff (PAS) staining on evaluating acute hepatic injury induced by carbon tetrachloride (CCl4) in rats.Methods：Rats were randomly divided into 2 groups： normal group and model group. Except normal rats, all the other rats in model group were injected subcutaneously 100% CCl4 3mL/kg once, then 50% CCl4 2mL/kg two times a week for 4 times. All the rats were sacrificed on the 15th day. Liver body ratio of rats was calculated; The serum parameters of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil), albumin (Alb) were assayed. Hepatic inflammation was observed with HE staining. Hepatic glycogen was stained by routine PAS staining and improved PAS staining. Results：Compared with the normal group, the activities of ALT and AST and TBil content increased significantly (P< 0.05). Alb content was significantly decreased compared with the normal group (P<0.05). HE staining showed that serious structural damage, hepatic steatosis and ballooning degeneration were seen in model group. Routine PAS staining showed that PAS positive staining area accounted for 32.38%±5.50% in normal group. Compared with the normal group, PAS positive staining area decreased significantly (8.60%±3.34 %, P< 0.01).Improved PAS staining showed that PAS positive staining area accounted for 75 .50%±9.02 % in normal group. Compared with the normal group, Improved PAS positive staining area decreased significantly (17.61%±3.53%, P< 0.01). In normal group and model group, improved hepatic glycogen staining showed positive staining of PAS was significantly higher than that of routine hepatic glycogen staining (P<0.01). This suggests that the improved PAS staining of liver glycogen positive stained area more truly reflected the degree of acute liver injury.Conclusion：The improved of Periodic acid-schiff (PAS) staining method is helpful to improve the evaluation quality degree of acute hepatic injury. |
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