To observe the effect of FZFA combined with chemotherapy on life quality and immune function of advanced colorectal cancer patients.Methods：Sixty six patients were randomly divided into treatment group and control group. Patients in the control group were given FOLFOX4 chemotherapy and supportive treatment, and the treatment group using FZFA based on the above treatment. We compared two groups of patients with recent objective efficient and stable rate and quality of life; the percentage of Th17/Treg cells and the ratio of CD4+T lymphocyte and inflammatory cytokines expression levels in blood of the two groups.Results：The objective response and stable rate and quality of life changes, the percentage of Th17/Treg cells and the ratio of CD4+T lymphocytes in peripheral blood of the two groups, and the expression of peripheral blood and inflammatory cytokine levels had significant difference (P<0.05).Conclusion：FZFA combined with chemotherapy and chemotherapy for advanced colorectal cancer patients can improve immune function, alleviate the adverse reaction, improve the quality of life, but the former is obviously better than the latter.