| 引用本文:万谦1，2，姚奏英2，3，伍林涛4，陆华1，2.齐墩果酸诱导多种小鼠胚胎干细胞向生殖细胞方向分化研究[J].世界中医药,2015,10(02):. |
|
| 齐墩果酸诱导多种小鼠胚胎干细胞向生殖细胞方向分化研究 |
| Study on Oleanolic Acid Inducing Several Strains of Mouse Embryonic Stem Cells to Differentiation towards Germ Cells |
| 投稿时间：2014-05-30 |
| DOI：10.3969/j.issn.1673-7202.2015.02.021 |
| 中文关键词: 齐墩果酸 小鼠胚胎干细胞 体外分化诱导物 生殖方向分化 |
| English Keywords:Oleanolic acid Mouse embryonic stem cells In vitro differentiation inducer Differentiation towards germ cells |
| 基金项目:国家重点基础研究发展计划（973计划）“从不孕不育探讨“肾主生殖”理论的研究”的相关研究（编号：2010CB530403）；成都中医药大学实验技术项目(编号：063003)；成都中医药大学校基金(编号：ZRMS201248) |
|
| 摘要点击次数: 1025 |
| 全文下载次数: 1297 |
| 中文摘要: |
| 目的：寻找一种能诱导多种胚胎干细胞向生殖细胞方向分化的化合物，并以常用的诱导物维甲酸（Retinoic Acid,RA）作为阳性参照物。方法：分别培养小鼠胚胎干细胞1B10、D3、R1/E，诱导它们形成拟胚体，让拟胚体贴壁生长，加入特定化合物诱导贴壁细胞分化，诱导72 h后，提取被诱导细胞的RNA，再合成cDNA，最后利用实时荧光定量PCR检测各被诱导细胞中与生殖分化相关的基因的表达水平的变化。结果：发现齐墩果酸（Oleanolic Acid，OA）显著上调了所有被研究的小鼠胚胎干细胞的生殖分化关键基因的表达水平，同时发现阳性参照物RA仅能诱导1B10、R1/E，而不能诱导D3向生殖细胞方向分化。结论：OA能诱导多种胚胎干细胞向生殖细胞方向分化，诱导能力方面具有比常用诱导物维甲酸更广泛的普遍性。 |
| English Summary: |
| To find an agent which can induce several strains of embryonic stem cells to differentiate towards germ cells,with the common inducer retinoic acid (RA) as positive control.Methods：Different strains of mouse embryonic stem cells (mESC) 1B10,D3,R1/E were respectively cultured,and induced to form embryoid bodies (EBs) which were allowed to attach; different compounds were then added to intervene the attached cells; the intervene continued for 72 h and RNA were extracted and cDNA were synthesized; finally,real-time fluorescence quantitative PCR (qPCR) assay were used to measure the transcriptional expression patterns of 11 reproductive differentiation related genes.Results：It was found that oleanolic acid (OA) can induce all 3 strains of mouse mESC to differentiate towards germ cells and the positive control RA can only induce mESC-1B10 and mESC-R1/E to differentiate towards germ cells.RA cannot induce mESC-D3 to differentiate towards germ cells.Conclusion：OA can induce several strains of embryonic stem cells to differentiate towards germ cells and it has broader universality than the positive control RA. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
