| 引用本文:艾斯1，2，3，郑健1，2，3，林青2，3，邱彩霞1，2，林雄2，宋艳芳2.肾康灵调控系膜细胞炎性反应因子的机制研究[J].世界中医药,2015,10(02):. |
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| 肾康灵调控系膜细胞炎性反应因子的机制研究 |
| Study on Mechanism of Shenkangling Regulating Inflammatory Factor of Mesangial Cells |
| 投稿时间：2014-05-09 |
| DOI：10.3969/j.issn.1673-7202.2015.02.022 |
| 中文关键词: 肾康灵 系膜细胞 氧化低密度脂蛋白 CXCL16 CD36 |
| English Keywords:Shenkangling Mesangial cells Oxidized low-density lipoprotein CXCL16 CD36 |
| 基金项目:本课题由福建省自然科学基金面上项目(编号：2011J01199);国家自然科学基金青年基金项目(编号：81202835);福建省卫生厅青年基金项目(编号：2012-1-30) |
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| 中文摘要: |
| 目的：观察氧化低密度脂蛋白(Oxidized Low-Density Lipoprotein，ox-LDL)对系膜细胞(Mesangial Cells，MCs)分泌炎性反应递质功能的影响，并从细胞分子生物学水平阐明肾康灵的作用机制。方法：采用肾康灵干预增殖的系膜细胞，并利用分子生物学技术检测CXCL16、CD36、IFN-γ、IL6、TNF-α含量或基因水平。结果：利用ox-LDL诱导大鼠系膜细胞增殖并加入CXCR6受体后，CXCL16、CD36、IFN-γ、IL6、TNF-α的含量或基因水平显著升高，肾康灵呈浓度依赖性降低其表达水平。结论：ox-LDL诱导系膜细胞增殖时通过CXCR6介导，可促进CXCL16、CD36、IFN-γ、IL6、TNF-α等炎性反应递质的释放，中药肾康灵可通过抑制炎性反应递质的释放，保护系膜细胞的功能。 |
| English Summary: |
| To clarify the mechanism of Shenkangling on mesangial cells and to observe oxidized low density lipoprotein(ox-LDL) induced Mesangial Cells(MCs) secretion of inflammatory cytokine.Methods：MCs were treated with Shenkangling,and the levels of the inflammatory cytokines,such as CXCL16,CD36,IFN-γ,IL6,TNF-αby ELISA,Real Time-PCR and Western blot were detected.Results：The levels of CXCL16,CD36,IFN-γ,IL6,TNF-α were significantly increased after CXCR6 joined and ox-LDL induced mesangial cell proliferation,then all levels were significantly reduced by Shenkangling in a concentration dependent manner.Conclusion：ox-LDL may promote the release of inflammatory mediators such as in mesangial cells,and CXCR6 may mediate this path.Shenkangling has effects for ameliorating ox-LDL-induced MC proliferation and inflammatory reactions. |
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