To investigate the effect and mechanism of Chinese herbal compounds prescription, which based on the traditional therapeutics of “expelling stone, diuresis and removing stasis”, on uric acid (UA) in rats with hyperuricemia. Methods: Male Wistar rats were randomly divided into normal group, model group, Tongfengding Capsules group, Benzbromarone group and Chinese herbal compounds prescription group(TFK) in two doses of 6.07 g·kg-1 and 3.03 g·kg-1. In addition to the normal group, the rest were offered with Ademine and the Ethambutol for 10 days. While the appropriate drugs administered to the rats of the administration group, the normal group and the model group were given by gavage distilled water for 10 days. Then blood was collected, UA, BUN content and XOD activity in serum were determined by ultraviolet–visible spectroscopy while UAT、OAT content in serum were detected by radiommunoassay. Results: Compared with the model group, the concentration of UA in high-dose and middle-dose Chinese herbal compounds prescription group significantly decreased in serum(P<0.05,P<0.01). The level of XOD activity of high-dose group significantly decreased as compared to the model group (P<0.01). The BUN content of middle-dose group was decreased in serum compared to the model group (P<0.05). The UAT content of high-dose group compared to the model group was significantly increased (P<0.01). The content of OAT in high-dose and middle-dose group was significantly increased in serum compared to the model group (P<0.01). Conclusion: The Chinese herbal compounds prescription which based on the traditional therapeutics of “expelling stone, diuresis and remove stasis” decreased the uric acid content in hyperuricemia model rats. The mechanism may be related to the relieving effect of the activation of XOD, reducing generation of uric acid, protecting renal function, increasing of UAT and OAT content and promoting excretion of uric acid.