| 引用本文:冯英楠1，陈菲2，张鹏2，庄伟2，林晓兰2，王秀娟1.优化治疗神经胶质瘤复方抗瘤丸的实验研究[J].世界中医药,2015,10(03):. |
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| 优化治疗神经胶质瘤复方抗瘤丸的实验研究 |
| Study on Optimization of KLW for Treating Glioma |
| 投稿时间：2014-12-01 |
| DOI：10.3969/j.issn.1673-7202.2015.03.026 |
| 中文关键词: 抗瘤丸 神经胶质瘤 处方优化 |
| English Keywords:KLW Glioma Prescription optimization |
| 基金项目:北京市中医药科技项目(编号:JJ2010-12);卫生局215人才-领军人才(编号:303-01-005-0063);市科委-基地培育与发展工程专项(编号:303-01-004-0004-02);首都医科大学基础临床课题(编号:13JL71) |
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| 中文摘要: |
| 目的:通过实验研究,优化治疗神经胶质瘤中药复方抗瘤丸(KLW)的组成,得出最佳处方配伍。方法:采用大鼠神经胶质瘤C6细胞BALB/c裸鼠移植瘤模型,将模型裸鼠按瘤块体积随机分为10组(模型组,阳性药对照组(替莫唑胺TMZ组),KLW-1、KLW-2、KLW-3、KLW-4高、低剂量组,每组7只裸鼠。)连续给药15 d,检测各组裸鼠体重、瘤块体积、瘤块重量、微血管密度(MVD)、血管内皮生长因子(VEGF)、凋亡细胞、Bcl-2,Bax蛋白表达水平。结果:与模型组比较,KLW-1高、低剂量组,KLW-2、KLW-4高剂量组,TMZ组瘤体积显著减小(P<0.05);KLW-4高剂量组,TMZ组移植瘤的MVD值明显降低(P<0.05);KLW-2、KLW-4高剂量组,TMZ组移植瘤VEGF灰度值明显增高(P<0.05);KLW-1、KLW-2、KLW-4高剂量组,TMZ组细胞凋亡率显著增高（P<0.05);各给药组Bcl-2/Bax的比例增加。结论:处方KLW-1高、低剂量组,KLW-2、KLW-4高剂量组对C6移植瘤的生长具有较好的抑制作用。 |
| English Summary: |
| To optimize compound KLW, and screen for the best prescription. Methods: C6 cells in BALB/c nude mice transplantation tumor model was established, and then randomly divided into ten groups (the model control group, the TMZ group, and low, high dose KLW-1, KLW-2, KLW-3, KLW-4 group, each group including seven rats). The rats were orally administered with medication for 15days. The study measured weight of mice, the transplanted tumor volume and weight, MVD, VEGF, apoptotic cells, the Bcl - 2, Bax protein expression level. Results: Compared with model control group, low, high dose KLW-1, high dose KLW-2 and KLW-4 TMZ group of tumor volume significantly reduced（P<0.05）; high dose KLW-4, TMZ group of tumor MVD decreased (P<0.05); high dose KLW-2 and KLW-4， TMZ group of tumor VEGF value increased (P<0.05); high dose KLW-1,KLW-2 and KLW-4, TMZ group of cell apoptosis rate increased (P<0.05); each dose group the Bcl - 2 / Bax ratio increases. Conclusion: Low and high dose of KLW-1and KLW-2, high dose KLW-4 have good effects on the growth of C6 transplantation tumor inhibition. |
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