Abstract:To optimize compound KLW, and screen for the best prescription. Methods: C6 cells in BALB/c nude mice transplantation tumor model was established, and then randomly divided into ten groups (the model control group, the TMZ group, and low, high dose KLW-1, KLW-2, KLW-3, KLW-4 group, each group including seven rats). The rats were orally administered with medication for 15days. The study measured weight of mice, the transplanted tumor volume and weight, MVD, VEGF, apoptotic cells, the Bcl - 2, Bax protein expression level. Results: Compared with model control group, low, high dose KLW-1, high dose KLW-2 and KLW-4 TMZ group of tumor volume significantly reduced(P<0.05); high dose KLW-4, TMZ group of tumor MVD decreased (P<0.05); high dose KLW-2 and KLW-4, TMZ group of tumor VEGF value increased (P<0.05); high dose KLW-1,KLW-2 and KLW-4, TMZ group of cell apoptosis rate increased (P<0.05); each dose group the Bcl - 2 / Bax ratio increases. Conclusion: Low and high dose of KLW-1and KLW-2, high dose KLW-4 have good effects on the growth of C6 transplantation tumor inhibition.