Acupuncture has a good effect on treating extrahepatic biliary system diseases. With deepening basic research, disorder of motor function of biliary system is considered as an important factor of all kinds of biliary system diseases. Combining the retrieval and analysis of both domestic and international literature on regulation mechanism of acupuncture for motor function of extrahepatic biliary system with experimental results, this paper studied regulation mechanism of acupuncture for nerve and humor of motor function of extrahepatic biliary system, regulation mechanism of acupuncture for oddi sphincter, and the influence of acupuncture on gallbladder pressure and tension etc., aiming to provide thoughts for future research and practical significance for clinical acupuncture.