| 引用本文:杨堃.小儿感冒颗粒的质量标准研究[J].世界中医药,2015,10(03):. |
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| 小儿感冒颗粒的质量标准研究 |
| Study of Quality Standard of Xiao’er Ganmao Granules |
| 投稿时间：2014-04-26 |
| DOI：10.3969/j.issn.1673-7202.2015.03.032 |
| 中文关键词: 小儿感冒颗粒 质量标准 TLC HPLC 连翘苷 |
| English Keywords:Xiao’er Ganmao Granules Quality standards TLC HPLC Phillyrin |
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| 中文摘要: |
| 目的：建立小儿感冒颗粒的质量标准。方法：用TLC法鉴别了小儿感冒颗粒中广藿香、菊花、连翘和用HPLC法测定了小儿感冒颗粒中连翘苷的含量；色谱柱为DiamonsilTM C18柱（5 μm，4.6 mm×250 mm），柱温为30 ℃，以乙腈-水（24∶76）为流动相，检测波长为202 nm，流速1.0 mL/min。结果：在TLC色谱中均能检出百秋李醇、菊花、连翘苷；且特征斑点明显，阴性无干扰；连翘苷在0.023 62 μg～0.590 5 μg范围内呈良好的线性关系，r＝0.99999（n=6），平均回收率为99.06%，RSD＝1.5%.结论：方法简便、准确、重现性好；可用于小儿感冒颗粒的质量控制。 |
| English Summary: |
| To establish the quality standards for Xiao’er Ganmao Granules. Methods: Pogostemonis herba, chrysanthemi flos, and forsythiae fructus were identified with TLC. Phillyrin was meatured by HPLC. DiamonsilTM C18 (5 μm，4.6 mm×250 mm) column was used. The column temperature was at 30 ℃. Acetonitrile-water (24∶76) was used as a mobile phase. The detection wavelength was at 202 nm. The flow rate was 1.0 mL/min. Results: Patchouli alcohol, chrysanthemi flos, and forsythiae could be detected by TLC, with the feature spot obvious, negative interference-free. Phillyrin showed a good linear relationship at the range of 0.02362～0.5905μg，r=0.99999 (n=6)，The average recovery rate was 99.06％ and RSD was 1.5％. Conclusion: TLC method proves to be simple, accurate and with good reproducibility, thus can be used for quality control of Xiao，er Ganmao Granule. |
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