| 引用本文:李秀荣，齐元富，李慧杰.芪连扶正胶囊对TGF-β介导EMT作用的影响[J].世界中医药,2015,10(04):. |
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| 芪连扶正胶囊对TGF-β介导EMT作用的影响 |
| Effect of Qilian Fuzheng Capsule on TGF-β-mediated EMT |
| 投稿时间：2013-08-30 |
| DOI：10.3969/j.issn.1673-7202.2015.04.022 |
| 中文关键词: 芪连扶正胶囊 TGF-β EMT 抗肿瘤转移 |
| English Keywords:Qilian Fuzheng Capsule TGF-β EMT Anti-metastatic |
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| 中文摘要: |
| 目的：观察芪连扶正胶囊含药血清对TGF-β及EMT相关分子在肺癌A549细胞中表达的影响，探讨相关机制。方法：体外培养肺癌A549细胞，制备芪连扶正胶囊含药血清，分组干预，采用RT-PCR检测TGF-β1mRNA表达，IC检测E-cad与N-cad表达。结果：对照组、芪连扶正低剂量、中剂量、高剂量组、化疗组及联合组的TGF-β1 mRNA相对表达量依次为（31.11±1.58）%、（29.53±1.50）%、（27.28±1.75）%、（25.23±1.38）%、（23.03±1.59）%、（20.72±1.38）%；各组E-cad、N-cad均有不同程度阳性表达，除芪连扶正胶囊低剂量组外，各药组E-cad阳性率均高于对照组、N-cad则相反(P<0.05或P<0.01)；与化疗组相比，联合组及芪连扶正胶囊各组亦有统计学意义(P<0.05或P<0.01)。结论：芪连扶正胶囊可通过逆转TGF-β介导的EMT作用防止肿瘤转移。 |
| English Summary: |
| To observe the effect of Qilian Fuzheng Capsule on expression of EMT(epithelial mesenchymal transition) and TGF-β in lung cancer A549 cells, and study its possible mechanism.Methods:A549 cells were cultured in vitro and group intervened, RT-PCR to detected the expression of TGF-β1mRNA, using Immunohistochemical to detected the expression of E-cad and N-cad.Results:the TGF-β1 mRNA relative expression of the control group，Qilian Fuzheng Capsule low, medium and high dose groups, chemotherapy group and combined group were （31.11±1.58）%、（29.53±1.50）%、（27.28±1.75）%、（25.23±1.38）%、（23.03±1.59）%、（20.72±1.38）% respectively. Except the low-dose group, the expression of E-cad and N-cad in other drug groups were all significantly different compared with the control group; the combined group and all Qilian Fuzheng Capsule groups were significantly different compared with the chemotherapy group.Conclusion:Qilian Fuzheng Capsule can reverse the TGF-β-mediated EMT therefore play an anti-tumor effect. |
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