| 引用本文:戚晴雪1，马玉峰2，吴忌1，郑皓云1，朗松1，杨黎黎1，赵军1，张栋1，王庆甫2.低频超声促透通络止痛中药对不同程度膝骨关节炎大鼠模型CTX-Ⅱ、COMP及MMP-3的影响[J].世界中医药,2015,10(04):. |
|
| 低频超声促透通络止痛中药对不同程度膝骨关节炎大鼠模型CTX-Ⅱ、COMP及MMP-3的影响 |
| Effect of CTX-Ⅱ,COMP and MMP-3 on Rat Models with Knee Osteoarthritis by Tongluozhitong Herbs Through Low-Frequency Ultrasound Transdermal Penetration |
| 投稿时间：2014-06-11 |
| DOI：10.3969/j.issn.1673-7202.2015.04.024 |
| 中文关键词: 膝骨性关节炎 低频超声 通络止痛中药 软骨基质 软骨组织形态学 |
| English Keywords:Knee osteoarthritis Low-frequency ultrasound Tongluozhitong herbs Cartilage matrix Cartilage morphological |
| 基金项目:2012年北京市中医药外治法项目（编号：303-02-71） |
|
| 摘要点击次数: 1250 |
| 全文下载次数: 1140 |
| 中文摘要: |
| 目的：探讨通络止痛中药在低频超声促透下对大鼠不同程度膝骨关节炎（Knee Osteoarthritis，KOA）模型的软骨组织形态学及血清中CTX-Ⅱ、COMP及MMP-3的影响，阐述该疗法的作用机制及其优势。方法：采用切断前交叉韧带的经典方法制备KOA大鼠模型。SD大鼠随机分为3组：空白对照组、超声促透组和中药外敷组。所有大鼠左膝造模，空白对照组正常饲养，术后2、4、8周时随机选取空白对照组大鼠检测血清相关细胞因子；处死后观察膝关节股骨内侧髁软骨组织形态学改变，根据软骨改良Mankin’s评分予以评价，并根据Mankin’s评分结果确定大鼠KOA的病变程度。超声促透组和中药外敷组在术后2、4、8周时各随机选取大鼠进行干预处理，超声促透组使用超声电导仪对术侧膝关节促透通络止痛中药，中药外敷组仅使用中药外敷。1次/d，30 min，连续治疗2周。2周后处死，记录数据同空白对照组。结果：空白对照组大鼠造模后2、4、8周时大鼠软骨组织形态学发生明显改变，Mankin’s评分的增加与时间成正相关，血清中CTX-Ⅱ、COMP、MMP-3的含量发生不同程度的增高。超声促透组与空白对照组相比，软骨组织形态学发生改变，Mankin’s评分2、4、8周组降低，2、4周时差异有统计学意义（P<0.01），4周时有统计学意义（P<0.05）；其作用显著优于中药外敷组，2周时下降明显，两两相比有统计学意义（P<0.05）。超声促透组与空白对照组相比，2、4、8周时CTX-Ⅱ含量明显降低，二者相比有统计学意义（P<0.05）；8周时，CTX-Ⅱ含量降低，二者相比有统计学意义（P<0.05）；其作用显著优于中药外敷组。超声促透组与空白对照组相比，2、4周时COMP含量减少（P<0.05）；与中药外敷组相比，4周时含量降低明显，二者相比有统计学意义（P<0.05）。超声促透组和空白对照组相比，2、4周时MMP-3含量降低，二者有统计学意义（P<0.05）；4周时超声促透组与空白对照组相比，MMP-3含量显著降低（P<0.01），与中药外敷组相比，含量降低明显，二者相比有统计学意义（P<0.05）。结论：低频超声促透通络止痛中药可以有效减缓或阻止膝关节软骨的退行性变，这可能是通过降低血清中软骨基质相关细胞因子CTX-Ⅱ、COMP和MMP-3含量，减缓软骨基质的退变所致，但对重度KOA软骨退变的缓解作用并不明显；与传统的中药外敷法相比，具有起效快，疗效好等优点。 |
| English Summary: |
| To investigate effect of cartilage morphology and CTX-Ⅱ,COMP and MMP-3 in serum on varying degrees of knee osteoarthritis in rats treated with Tongluozhitong herbal with low-frequency ultrasound transdermal penetration and explain the mechanism and advantage of the therapy.Methods: The rat models of KOA were built by the classic method of cutting off anterior cruciate ligament.SD rats were randomly divided into three groups: control group,the group of ultrasonic transdermal penetration and herbal topical groups.Left knees of rats were models.The control group were normally fed,and were randomly selected to extract serum and detect cytokines at 2nd/4th/8th weeks after surgery and the control group were observed on femoral condylar cartilage morphology and were evaluated by cartilage Mankin's score.Ultrasonic transdermal penetration group and herbal topical group were randomly selected for intervention treatment at the2nd/4th/8th weeks after surgery,1/d,30min,2 weeks.After 2 weeks,the data was recorded which was the same as the control group.Results: Cartilage morphology of the control group were changed significantly at 2nd/4th/8th weeks after modeling and cartilage Mankin's score was positively correlated with increased time and CTX-Ⅱ,COMP,MMP-3 in serum increased.Compared with the control group,cartilage morphology and Mankin's score at 2nd/4th weeks changed (P<0.05).Its role was better than topical medicine group:.Mankin's score decreased significantly at 2nd week(P<0.05).Compared with the control group,CTX-Ⅱof ultrasonic transdermal penetration group decreased sharply at 2nd/4th/8th weeks (P<0.05),and it was significantly better than herbal topical groups.Compared with the control group,COMP of ultrasonic transdermal penetration group decreased sharply at 2nd and 4th weeks (P<0.05).Compared with the herbs topical group,COMP of ultrasonic transdermal penetration group decreased at 4th week (P<0.05).Compared with the control group,MMP-3 of ultrasonic transdermal penetration group decreased at 2nd/4th weeks and there was diversity(P<0.05).Compared with the herbs topical group,MMP-3 of ultrasonic transdermal penetration group decreased at 4 weeks (P<0.05).Conclusion: The low-frequency ultrasound transdermal Penetration Tongluozhitong herbs can effectively Preventing and slowing the Progression of the disease.It may be reducing cartilage matrix-associated cytokines CTX-Ⅱ,COMP and MMP-3 in serum,slowing the degeneration of cartilage matrix; but there is no obvious effect on cartilage of severe KOA.Compared with the traditional method of herbs topical,it has the advantages of rapid onset and efficacy. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
