世界中医药

目的:观察痛泻要方对腹泻型肠易激综合征(D-IBS)实验大鼠结肠运动指数(每分钟收缩强度曲线下面积，MI)和收缩频率(每分钟收缩次数,CF)的影响。方法:将SPF级新生SD雄性大鼠随机分为正常组、模型组、中药痛泻要方组。除正常组以外,其他2组均采用母婴分离加束缚应激的方法进行造模。第60天造模成功后,于第61天开始,正常组和模型组给予生理盐水,中药组给予4.92 g/100 g痛泻要方煎剂。3组均采用灌胃的方法给药,给药体积均为2 mL/(100 g·d),连续14 d。造模及给药过程中观察大鼠粪便Bristol分级评分及粪便含水量。采用离体张力测定灌流系统,测量3组大鼠离体结肠纵型平滑肌肌条(CLSMs)运动指数(MI,mg/min)和收缩频率(CF,times/min)的变化。结果:给药前,中药组和模型组粪便Bristol评分、含水量均高于正常组(P<0.05);给药后,中药组粪便Bristol评分及含水量均低于模型组(P<0.01)。给药前,中药组和模型组的运动指数(MI)、收缩频率(CF)结果近似,且均高于正常组(P<0.01);给药后,中药组的MI、CF均呈下降趋势,且低于模型组(P<0.01),与正常组比较无统计学意义(P>0.05)。结论:痛泻要方可有效改善D-IBS大鼠的腹泻症状,其作用机制可能是通过降低结肠平滑肌的运动指数及收缩频率实现的。

English Summary:

To observe the effects of Tong-Xie-Yao-Fang (TXYF) formula on movement index (MI, area under the curve of contraction intensity/min) and contraction frequency (CF, number of contractions/min) of experimental rats with diarrhea-predominant irritable bowel syndrome(D-IBS). Methods: The newborn Specific Pathogen Free(SPF) and Sprague-Dawley (SD) male pups were randomly divided into normal group, NMS+RS group and TXYF formula group. In addition to the normal group, D-IBS model with the method of neonatal maternal separation plus restraint stress (NMS+RS) were established in other two groups. On the 61st day, after the success of molding, normal group and NMS+RS group was given with saline, while TXYF formula group were given with 4.92 g/100 g TXYF formula. Three groups were dosed with the method of gavage with the drug delivery volume of 2 mL/100 g/d, for 14 consecutive days. The scores of Bristol classification and water content of the fecal pellets of rats were observed in the process of model building and dosing. The distinctions of movement index (MI, area under the curve of contraction intensity/min, mg/min) and contraction frequency (CF, number of contractions/min, times/min) of isolated colonic longitudinal smooth muscle strips (CLSMs) in three groups before and after treatment were observed with a Power Lab System. Results: Before treatment, scores of Bristol classification and water content of the fecal pellets in both TXYF formula group and NMS+RS group were all higher than that in normal group(P<0.05); After treatment, the scores of Bristol classification and water content of the fecal pellets in TXYF formula group were lower than that in NMS+RS group (P<0.01).Before treatment, the MI and CF of CLSMs in the NMS+RS and TXYF formula groups were similar and all higher than that in the normal group (P<0.01, respectively). After treatment, the MI and CF in the TXYF formula group was lower than in the NMS+RS group (P<0.01) and similar to the normal group (P>0.05). Conclusion: TXYF formula can effectively improve symptoms of D-IBS rats. Its mechanism may be achieved through Lower colon movement index and contraction frequency.

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