| 引用本文:张晨1，黄进2，詹菲1，张进1.黄芪多糖对四氯化碳诱导的大鼠肝纤维化的保护作用[J].世界中医药,2015,10(06):. |
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| 黄芪多糖对四氯化碳诱导的大鼠肝纤维化的保护作用 |
| Protective Effect of Astragalus Polysaccharide on Liver Fibrosis Induced by Carbon Tetrachloride in Rats |
| 投稿时间：2014-08-01 |
| DOI：10.3969/j.issn.1673-7202.2015.06.022 |
| 中文关键词: 肝纤维化 黄芪多糖 四氯化碳 |
| English Keywords:Liver fibrosis Astragalus polysaccharides Carbon tetrachloride |
| 基金项目:广州中医药大学高等学校博士学科点专项科研基金项目（编号：20114425120015） |
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| 中文摘要: |
| 目的：观察黄芪多糖（APS）对四氯化碳（CCl4）致大鼠肝纤维化的治疗作用。方法：采用CCl4诱导大鼠肝纤维化模型，造模成功后，取大鼠血清和肝组织进行检测。测定血清肝纤维化四项、肝脏组织中羟脯氨酸（HYP）含量；HE染色比较炎症及肝纤维化程度；免疫组织化学法分析肝组织中I型胶原（Col-I）、平滑肌肌动蛋白α(α-SMA)表达量。结果：与模型组相比，APS中、高剂量组的肝纤四项水平、炎症及肝纤维化程度明显降低（P<0.05）；APS中、高剂量组肝脏组织中HYP含量显著降低（P<0.01）；APS中剂量组Col-I、α-SMA表达显著减少（P<0.01）；APS高剂量组Col-I、α-SMA表达明显减少（P<0.05）。结论：APS对大鼠肝纤维化具有一定的防治作用，作用机制可能与其抑制Col-I、α-SMA表达有关。 |
| English Summary: |
| To study the protective effect of the Astragalus polysaccharides (APS) on liver fibrosis in rats caused by carbon tetrachloride (CCl4).Methods:CCl4 was used to induce rat liver fibrosis model. After making the model successfully, rat serum and liver tissue were collected to test. The serum liver fiber four and hydroxyproline (HYP) content in the liver tissue was tested; HE staining of liver tissue was used to compare inflammation and degree of liver fibrosis; expression of Col-I and α-SMA with immunohistochemical method was analyzed.Results:Compared with the model group, four levels of liver fibrosis, inflammation and liver fibrosis of APS high and medium groups were obviously lower(P<0.05); HYP content of APS high and medium groups were significantly lower (P<0.01); Col-I and α-SMA expression of APS medium group was significantly lower (P<0.01); Col-I and α-SMA expression of APS high group was obviously lower (P<0.05).Conclusion:The APS is effective to some extent in preventing liver fibrosis of rat, the functional mechanism may be related to limiting the expression of Col-I and α-SMA. |
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