| 引用本文:赵任杰1，吴丹彤2，李均2，姚兰2.丹酚酸A、B及其分子药对配伍对肾纤维化过程中CTGF及Par-3的干预作用[J].世界中医药,2015,10(06):. |
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| 丹酚酸A、B及其分子药对配伍对肾纤维化过程中CTGF及Par-3的干预作用 |
| Intervention Effect of Salvianolic acid A, B on CTGF and Par-3 Expression in the Process of Renal Fibrosis |
| 投稿时间：2014-12-23 |
| DOI：10.3969/j.issn.1673-7202.2015.06.024 |
| 中文关键词: 肾纤维化 丹酚酸A 丹酚酸B CTGF Par-3 |
| English Keywords:Renal interstitial fibrosis Salvianolic acid A Salvianolic acid B CTGF Par-3 |
| 基金项目:国家自然科学基金项目丹酚酸A、B、C分子药对配伍对肾纤维化过程中PDGF-C/PDGFR-α信号途径的干预作用(编号：81260603) |
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| 中文摘要: |
| 目的：观察丹酚酸A、B及其分子药对配伍对肾纤维化中CTGF及Par-3表达的影响。方法：采用50只雄性SPF级SD大鼠随机分为5组：正常组、造模组、丹酚酸A组、丹酚酸B组及丹酚酸A+B组。除正常组外，其余大鼠均造成UUO模型。各组予相应药物治疗2周，腹主动脉采血检测大鼠Cr、BUN；免疫荧光观察大鼠肾组织CTGF、Par-3表达情况。结果：1）肾功能检测：模型组大鼠血清Cr，BUN水平显著高于正常组（P<0.05）。丹酚酸A组大鼠血清Cr水平明显低于模型组（P<0.05）；丹酚酸A组和丹酚酸B组大鼠血清BUN水平明显低于模型组（P<0.05）；各治疗组间比较，血清Cr和BUN水平差异无统计学意义（P>0.05）。2）CTGF免疫荧光检测：和正常组相比，模型组大鼠肾组织肾小管上皮细胞阳性细胞数目明显增多，荧光强度明显增强（+++）；经丹酚酸A、B及组分配伍治疗后，CTGF荧光强度较模型组明显减弱，其中以丹酚酸A+B组减弱较为明显（±），丹酚酸B组次之（+）。3）Par-3免疫荧光检测结果：模型组大鼠肾组织肾小管上皮细胞阳性细胞数目比正常组明显减少，荧光强度明显减弱（±）；经丹酚酸A、B及组分配伍治疗后，丹酚酸A、B、A+B组Par-3荧光强度较模型组明显增强，其中以丹酚酸A+B组增强较为明显（+++），丹酚酸B组次之（++）。结论：经丹酚酸A、B及其分子药对配伍治疗后，可一定程度改善大鼠肾功能，但无统计学意义；丹酚酸组分配伍组能显著抑制大鼠肾组织CTGF的表达、促进Par-3的表达，其效果优于丹酚酸A和丹酚酸B组。该机制可能与其促进Par-3在UUO大鼠肾组织中的表达和减少CTGF的表达有关。 |
| English Summary: |
| To investigate the intervention effect of Salvianolic acid A, B and their component molecules of drug compatibility on expression of CTGF and Par-3 in the process of renal fibrosis. Methods: A total of 50 male Sprague-Dawley rats were randomly divided into five groups: normal group (n=10), the control group (n=10), salvianolic acid A group (n=10), salvianolic acid B group (n=10) and salvianolic acid A and B group (n=10). All rats were made into unilateral ureteral obstruction (UUO) model except the normal group. After two weeks of treatment, we took blood sample from abdominal aorta and examined Cr and BUN levels; and observed CTGF, Par-3 protein expression in kidney tissue by immunofluorescence. Results: 1)Results of renal function test: model groups serum Cr level was significantly higher than the normal group (P<0.05); the Sal A groups serum Cr level was obviously lower than the model group (P<0.05); the serum BUN levels in the Sal group A and group B significantly lower than the model group; among the treatment groups, serum Cr and BUN levels showed no significant difference (P> 0.05). 2)Results of CTGF immunofluorescence test: the number of rat renal tubular epithelial cells showing positive in model group significantly increased compared to the control group and the fluorescence intensity significantly enhanced (+++); after treatment of Sal A, B, and component compatibility, CTGF fluorescence intensity was reduced compared with the model group , among which the Sal A+B group decreased significantly (±) and followed by the Sal B group (+). 3)Results of Par-3 immunofluorescence test: the number of positive cells in rat renal tubular epithelial cells in the model group was significantly decreased than the normal group and the fluorescence intensity was significantly reduced (±); after treatment of Sal A, B and component compatibility, Par-3 the fluorescence intensity in the Sal A, Sal B, Sal A+B group was significantly enhanced compared with the model group , among which the Sal A+B group enhanced most obviously (+++), and followed by the Sal group B (++). Conclusion: Sal A, B and component compatibility treatment could partly improve rats renal function, but showed no significant difference. The Sal A+B group could significantly inhibit the expression of CTGF in renal tissue and promote expression of Par-3, and its effect was better than the Sal A group and Sal B group. It may be related to promoting the expression of Par-3 and reducing the expression of CTGF in UUO kidney tissue of rats. |
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