世界中医药

目的：观察含左归丸鼠血清对3T3-L1前脂肪细胞芳香化酶mRNA表达的影响。方法：将雌性育龄期大鼠分为正常组20只、假手术组17只、模型组8只、阳性对照组11只、左归丸高剂量组（左高组）6只、左归丸低剂量组（左低组）9只，连续灌胃11周，分离血清检测E2；以2%各类鼠血清加入培养液培养分化中的3T3-L1前脂肪细胞，48 h后测细胞上清液E2和细胞芳香化酶mRNA表达。结果：与模型组比较，左高组、左低组给药前后体重有升高趋势，但无明显统计学意义(P>0.05)；左高组、左低组鼠血清E2值较模型组无明显统计学意义(P>0.05)；培养3T3-L1前脂肪细胞48h后，细胞上清液E2值左高组（9.40±3.61）pg/mL、左低组（8.94±2.91）pg/mL比模型组（3.99±0.76）pg/mL略有升高；模型组（0.39）芳香化酶mRNA表达（P450arom/GAPDH）水平高于左高组(0.25)。结论：左归丸对去势雌鼠血清E2和体重无明显影响，可增强3T3-L1前脂肪细胞的芳香化酶活性。

English Summary:

To observe the effect of rat medicated serum containing Zuo Gui Pills on the expression of aromatase mRNA in 3T3-L1 adipocytes. Methods: Seventy seven female sexual matured SD rats were divided into normal control group (20), sham-operated group (17), model group(8), positive control group(11), high Zuo Gui Wan group(6), low Zuo Gui Pills group(9). They were fed with the corresponding medicines in the stomach for 11 weeks. Sera was separated to test E2; sera of 2% from different rats was mixed with 3T3-L1 adipocytes neutralized in nutrient solution for 48 hours and tested to measure E2 and expression of aromatase mRNA. Results: Compared with the model group, weight of rats in high Zuo Gui Pills group and low Zuo Gui Pills group increased but there was no significant difference(P> 0.05). The level of E2 in serum of rats in high Zuo Gui Pills group and low Zuo Gui Pills group had no significant difference(P> 0.05). After 3T3-L1 adipocytes was neutralized for 48 hours, E2 in sera was (9.40±3.61)pg/ml in high Zuo Gui Wan Pills and (8.94±2.91)pg/ml in low Zuo Gui Pills group, increasing slightly compared with the model group （3.99±0.76）pg/ml. The expression of aromatase mRNA of model group was P450arom／GAPDH which was higher than the high Zuo Gui Pills group. Conclusion: Zuo Gui Pills have no significant effects on weight and the level of E2 in serum of ovariectomized rats but can improve activity of aromatase in 3T3-L1 adipocytes.

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