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引用本文:胡国洪1，舒晴1，聂红海2，徐海英3.五味子对冰乙酸性胃溃疡寒、热证大鼠模型IL-8、VEGF水平的影响[J].世界中医药,2015,10(07):.

五味子对冰乙酸性胃溃疡寒、热证大鼠模型IL-8、VEGF水平的影响

The Impact of Schisandra Chinensis on the Expression of IL-8 and VEGF in Gastric Ulcer Rat Model Induced by Glacial Acetic Acid Combined with Cold or Heat Syndrome

投稿时间：2014-05-26

DOI：10.3969/j.issn.1673-7202.2015.07.023

中文关键词: 五味子胃溃疡冰乙酸涂抹寒热证 IL-8 VEGF

English Keywords:Schisandra chinensis Gastric ulcer Mearing glacial acetic acid Cold or heat syndrome IL-8 VEGF

基金项目:清远市科技计划项目医疗卫生项目（编号：00136071320529027）

作者	单位
胡国洪1，舒晴1，聂红海2，徐海英3	1 广州中医药大学附属清远医院/清远中医院病理科，广州，511500； 2 广州中医药大学附属清远医院/清远市中医院肛肠普外科，广州，511500； 3 广州中医药大学附属清远医院/清远市中医院病案统计室，广州，511500

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中文摘要:

目的：探讨五味子对冰乙酸性胃溃疡寒、热证大鼠模型白细胞介素-8（Interleukin-8，IL-8）、血管内皮生长因子（Vascular Endothelial Growth Factor，VEGF）表达水平的影响。方法：采用寒、热因素结合冰乙酸涂抹法建立大鼠胃溃疡寒、热证模型,通过HE染色观察组织病理改变，联合采用酶联免疫法、RTPCR、免疫组化方法检测受试大鼠血清IL-8、VEGF的含量变化。结果：五味子能够缩小冰乙酸胃溃疡寒热证模型的溃疡面积；冰乙酸涂抹型胃溃疡模型组血清及组织内的IL-8、VEGF含量显著升高，与空白组比较差异有统计学意义（P<0.01）。五味子可显著升高热证组、寒证组中VEGF的表达（P<0.01），同时下调热证组、寒证组中IL-8的表达（P<0.05）。五味子对胃溃疡面积的缩小作用与对IL-8及VEGF的调节作用呈显著相关性。结论：五味子能够改善冰乙酸性胃溃疡寒、热证大鼠模型胃溃疡黏膜面积，可能通过下调IL-8的表达，同时促进VEGF的表达有关。

English Summary:

To explore the impact of schisandra chinensis on the expression of IL-8 and VEGF in gastric Ulcer rats model induced by glacial acetic acid combined with cold or heat syndrome.Methods:Exposing the rats in cold or heat environment and applying glacial acetic acid to skin to establish gastric ulcer model combined with cold and heat syndrome. Using HE stain to observe the pathologic changes, using ELISA, RTPCR as well as Immunohistochemistry to detect the contents of IL-8 or VEGF both in serum and in stomach. Results:Schisandra chinensis diminishes the area of ulcer. Compared with the blank group, the contents of both IL-8 and VEGF increased remarkably in each ulcer model group without schisandra chinensis. (P<0.01). Schisandra chinensis can up-regulate the expression of VEGF while down-regulate the expression of IL-8 in both serum and stomach of heat-ulcer group or cold-ulcer group obviously ( P<0.05).Conclusion:The therapeutic action of Schisandra chinensis on heat-gastric ulcer or cold-gastric ulcer can be achieved by up-regulating the expression of VEGF while down-regulating the expression of IL-8 in both serum and stomach.

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