| 引用本文:罗云1，卢珊1，周平2，孙桂波1，孙晓波1.木犀草素改善高脂诱导的ApoE-/-小鼠非酒精性脂肪肝及动脉粥样硬化作用研究[J].世界中医药,2015,10(08):. |
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| 木犀草素改善高脂诱导的ApoE-/-小鼠非酒精性脂肪肝及动脉粥样硬化作用研究 |
| Effects of Luteolin on High Fat Diet-induced ApoE-/-mice With Non-alcoholic Steatohepatitis and Atherosclerosis |
| 投稿时间：2015-06-29 |
| DOI：10.3969/j.issn.1673-7202.2015.08.004 |
| 中文关键词: 木犀草素 非酒精性脂肪肝 动脉粥样硬化 氧化损伤 ApoE-/-小鼠 |
| English Keywords:Luteolin Non-alcoholic steatohepatitis Atherosclerosis Oxidative injury ApoE-/-mice |
| 基金项目:国家自然科学基金项目（编号：81374010）；国家科技重大专项课题，重大新药创制基金（编号：2012ZX09501001-004） |
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| 中文摘要: |
| 目的：探讨木犀草素对高脂诱导的ApoE-/-小鼠非酒精性脂肪肝及动脉粥样硬化的改善作用。方法：采用高脂饲料喂养诱导非酒精性脂肪肝和动脉粥样硬化模型，木犀草素低、高剂量（70,140 mg/kg）灌胃给药，每天1次，连续12周。给药结束后，检测血清血脂四项、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶（GSH-Px），肝脏匀浆检测谷丙转氨酶、天冬氨酸氨基转移酶(AST)；观察肝脏组织HE染色和油红O染色以及主动脉根部的油红O染色；通过TUNEL试验检测肝脏细胞的凋亡；应用免疫组化检测肝脏bcl-2和caspase-3的表达。结果：木犀草素能够使肝脏ALT、AST水平降低；提高血清中SOD、GSH-Px，降低血清MDA的表达；改善肝脏中脂质堆积以及脂肪泡的形成，减少主动脉根部的脂质堆积；降低TUNEL阳性细胞数，以及提高肝脏中bcl-2和减少caspase-3的表达。结论：本研究表明木犀草素可通过减少氧化损伤来改善高脂诱导的ApoE-/-小鼠非酒精性脂肪肝及动脉粥样硬化。 |
| English Summary: |
| This study was to explore the possible effects of luteolin on ApoE-/-mice with non-alcoholic steatohepatitis induced by high fat diet as well as the efficacy on atherosclerosis. Methods: The model was induced by high fat diet, and luteolin were administered by gastric perfusion at 70 and 140 mg/kg qd for 12 weeks. The serum level of superoxide dismutase(SOD), malondialdehyde(MDA), glutathion peroxidase(GSH-Px)were measured. The contents of alanine transaminase(ALT), aspartate aminotransferase(AST)were measured in liver tissue of grout. The pathological changes of liver tissue were examined by HE staining and the lipid accumulation in the liver and aortic root was tested by oil red O staining. The apoptotic hepatocytes were detected by TUNEL assay and the expression levels of Bcl-2 and caspase-3 were observed by immunohistochemisty. Results: The results showed that Luteolin(70, 140 mg/kg)improved the liver function significantly through reducing the level of ALT, AST, and increasing the content of SOD, GSH-Px and Bcl-2 while reducing the content of MDA, lipid accumulation, hepatic steatosis, TUNEL-positive cells and the expression of caspase-3. Conclusion: The results suggest that Luteolin may inhibit the liver injury and atherosclerosis induced by high fat diet by decreasing the oxidative stress. |
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