| To explore the protective effect of different polar fractions in total flavones of Clinopodium chinenseon on H9c2 myocardial cell damage induced by hypoxia/ reoxygenation(H/R). Methods: The hypoxia/ reoxygenation injury cardiomyocyte model was established and screened out parts with 10%（2#）, 20%（3#）, 30%（5#)of carbinol. Cell viability was determined by MTT method. We collected culture cell and its supernatant for detecting LDH, SOD, GSH-Px, CAT activity and MDA content. Results: In H/R group, after hypoxia 4 h and reoxygenation 24 h, the myocardial cell viability decreased significantly compared to control group. Compared with H/R group, 2#、3#、5#different isolated fragments in total flavones of Clinopodium chinense could increase cell viability. Compared to control group, SOD, GSH-Px, CAT were dropped remarkably, and LDH and MDA increased remarkably in H/R group. Compared to H/R group, 2#、3#、5#medication groups decreased LDH and MDA significantly, and increased SOD, GSH-Px, CAT significantly in a dose-dependent manner. Conclusion: 2#、3#、5# can reduce myocardial damage induced by hypoxia/ reoxygenation, the mechanism is related with the enhancement ability of myocardial cells to clear oxygen free radicals, and decrease the generation of lipid peroxide. |
