| 引用本文:杨培1，周红1，辛天怡1，马双姣1，段宝忠2，姚辉1.黄精属药用植物DNA条形码鉴定研究[J].世界中医药,2015,10(08):. |
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| 黄精属药用植物DNA条形码鉴定研究 |
| Identification Study of DNA Barcode Sequences in the Medicinal Plants of Polygonatum |
| 投稿时间：2015-06-29 |
| DOI：10.3969/j.issn.1673-7202.2015.08.010 |
| 中文关键词: 黄精属 分子鉴定 DNA条形码 |
| English Keywords:Polygonatum Molecular identification DNA barcode |
| 基金项目:国家科技支撑计划（编号：2011BAI07B08）；国家自然科学基金项目（编号：31460084） |
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| 中文摘要: |
| 目的：评价DNA条形码候选序列对黄精属药用植物的鉴定能力。方法：对44份黄精属样品应用通用引物扩增其叶绿体rbcL、matK、psbA-trnH及核ITS2和ITS序列，分析其种内种间变异和barcoding gap，应用BLAST和Nearest Distance方法计算物种鉴定效率。结果：ITS2和ITS序列通用引物扩增效率低，叶绿体matK序列获得率最低，rbcL最高，三条序列种内与种间变异均较小，无明显的barcoding gap。基于BLAST和Nearest Distance方法计算，三条叶绿体序列对黄精属药用植物的鉴定效率均较低。结论：DNA条形码候选序列psbA-trnH、matK及rbcL不适用于黄精属药用植物的鉴定，叶绿体全序列或通过叶绿体全序列筛选合适的DNA片段可能是该属药用植物分子鉴定的方向。 |
| English Summary: |
| To evaluate the identification efficiencies of the DNA barcode sequences for the medicinal plants of Polygonatum. Methods: The chloroplast psbA-trnH, matK, and rbcL and nuclear ITS and ITS2 of 44 Polygonatum samples were amplified using the universal primers. The intra-and inter-specific genetic distance and barcoding gap were analyzed. The identification efficiencies of the sequences were calculated by BLAST and Nearest Distance methods. Results: ITS and ITS2 of Polygonatum were difficult to obtain using the universal primers. The available rbcL were the highest while the psbA-trnH was the lowest. The intra-and inter-specific genetic distances were all low and no obvious barcode gap existed in three candidate loci. The identification efficiencies of the three chloroplast regions were all low according to BLAST and Nearest Distance methods. Conclusion: The DNA barcode sequences did not meet the demands of identifying the medicinal plants of Polygonatum, and the complete chloroplast genome sequences(CP)or the suitable regions screened from CP may be applied to identify the medicinal plants of Polygonatum in the future. |
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