| 引用本文:古丽娜尔·叶尔森那力1，木拉提·克扎衣别克1，2，3，江阿古丽·艾山3，江安努尔·这依肯3，地达尔·巴合提坚2,3.HPLC法同时测定哈医祛风膏中去氢骆驼蓬碱和骆驼蓬碱的含量及疗效观察[J].世界中医药,2015,10(08):. |
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| HPLC法同时测定哈医祛风膏中去氢骆驼蓬碱和骆驼蓬碱的含量及疗效观察 |
| Study of the Content and Effect of Harmine and Harmaline in Hayi Wind-dispelling Ointment by HPLC Method |
| 投稿时间：2015-03-10 |
| DOI：10.3969/j.issn.1673-7202.2015.08.029 |
| 中文关键词: 哈医祛风膏 去氢骆驼蓬碱 骆驼莲碱骆驼蓬碱 高效液相色谱法 疗效。 |
| English Keywords:Hayi wind-dispelling ointment Harmine Harmaline HPLC method Clinical efficacy |
| 基金项目:新疆自治区科研机构创新发展专项资金(编号:2013002);新疆地产中药民族新药研发项目(编号:XJHPHY-2010-YH) |
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| 中文摘要: |
| 目的:建立高效液相色谱法(HPLC)测定哈医祛风膏中去氢骆驼蓬碱和骆驼蓬碱的含量及临床疗效观察。方法:采用高效液相法测定含量,色谱柱为Agilent TC-C18(4.6 mm×250 mm,5 μm),以甲醇:0.01 moL/L硫酸铵溶液(37:63,用三乙胺调pH至4.0)为流动相,流速为1 mL/min,柱温:25 ℃,检测波长在320 nm。用完全随机法分实验组和对照组进行比较哈医祛风膏疗效。结果:去氢骆驼蓬碱的线性范围为12~83 μg(r=0.999 8,n=6)平均回收率为100.14%，RSD值为1.33%、骆驼蓬碱的线性范围为12~83 μg(r=0.999 8,n=6)平均回收率为98.89%,RSD值为1.77%。疗效比较治疗组的总有效率为97.3%、对照组的总有效率为90.1%,2组有显著性差异,P<0.01。表明治疗组总体疗效明显优于对照组。结论:高效液相色谱法方法简便,准确,可靠,可以用于哈医祛风膏中去氢骆驼蓬碱和骆驼蓬碱的含量测定。并哈医祛风膏治疗颈肩腰腿痛的效果较好、疗效确切、安全性良好,可降低患者复发率,可考虑在临床上推广。 |
| English Summary: |
| To study the content and clinical effect of Harmine and Harmaline in Hayi wind-dispelling ointment by HPLC method. Methods: The sample was separated on Agilent TC-C18(4.6 mm ×250 mm, 5 μm). CH30H of 0.01 moL·L-1 and(NH3)2SO4(63∶37 pH regulated to 4.0 by triethylamine)were used as the mobile phase, with flow rate at 1 mL·min-1, column temperature at 25 ℃, the determining wavelength at 320nm. Complete random method was used to divide control group and experimental group to compare the efficacy of Hayi wind-dispelling ointment. Results: Harmine calibration curve was linear over the range of 12-83 μg(r=0.9998，n=6). The average recovery rate was 100.14%, RSD was 1.33%. Harmaline calibration curve was linear over the range of 12-83 μg(r=0.9998, n=6). The recovery rate was 98.89%， RSD was 1.77%. The total effective rate of treatment group was 97.3%, followed by the control group at 90.1%.There was significant difference between the two groups(P<0.01), which indicates that the overall curative effect of treatment group was obviously better than that of the control group.Conclusion: The HPLC method is simple, accurate, and reliable, which can be applied to determine the content of harmine and harmaline in Hayi wind-dispelling ointment. Hayi wind-dispelling ointment is effective and safe for the treatment of shoulder, back and leg pain and can reduce the relapse rate of patients, thus can be further promoted in clinical practice. |
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