| 引用本文:杨先照，江锋，叶永安.抗纤抑癌方调控HGF/c-Met mRNA干预肝癌前病变的实验研究[J].世界中医药,2015,10(09):. |
|
| 抗纤抑癌方调控HGF/c-Met mRNA干预肝癌前病变的实验研究 |
| The Regulation Effect of Kangxian Yi-ai Formula on HGF and c-Met mRNA in Rats with Liver Precancerous Lesions |
| 投稿时间：2015-08-20 |
| DOI：10.3969/j.issn.1673-7202.2015.09.006 |
| 中文关键词: 抗纤抑癌方 肝癌前病变 肝细胞生长因子 c-Met |
| English Keywords:Kangxian Yi-ai formula Liver precancerous lesions Hepatic growth factor c-Met |
| 基金项目:北京中医药大学青年教师自主课题（编号：2009JYBZZ-JS078） |
|
| 摘要点击次数: 934 |
| 全文下载次数: 1161 |
| 中文摘要: |
| 目的：观察抗纤抑癌方对肝癌前病变大鼠HGF/c-Met mRNA的影响，探讨抗纤抑癌方防治肝癌前病变的作用机制。方法：75只SD雄性大鼠随机分为正常组、模型组、抗纤抑癌组、鳖甲软肝组和秋水仙碱组。以二乙基亚硝胺诱发大鼠肝癌前病变模型，用抗纤抑癌方进行干预，并以复方鳖甲软肝片、秋水仙碱片作为对照，采用免疫组化检测肝组织α-SMA的表达情况，实时荧光定量PCR检测各组大鼠肝脏的HGF和c-Met mRNA的表达量。结果：免疫组化显示，与模型组及秋水仙碱组比较，抗纤抑癌组能明显减少α-SMA表达量，差异有统计学意义（P<0.01，P<0.05）。实时荧光定量PCR结果显示，HGF mRNA的相对表达量抗纤抑癌组与模型组比较有统计学意义(P<0.05)，与秋水仙碱组比较亦有统计学意义(P<0.05)，抗纤抑癌方能显著提高肝癌前病变大鼠HGF mRNA的表达水平。c-Met mRNA在DEN造模的各组中均低表达，各组间差异无统计学意义（P>0.05）。结论：抗纤抑癌方能够提高肝癌前病变大鼠HGF mRNA的表达水平，这可能是其防治肝癌前病变的作用机制之一。 |
| English Summary: |
| To observe the effect of Kangxian Yi-ai formula on HGF and c-Met mRNA in rats with liver precancerous lesions， and thus to investigate its prevention and treatment mechanism. Methods： Seventy-five SD rats were randomly divided into normal group， model group， Kangxian Yi-ai(KXYA) group， Biejiaruangan(BJRG) group and colchicine group. Established the animal models of diethylnitrosamine(DEN)-induced liver precancerous lesions and used KXYA formula to intervene. BJRG tablets and colchicine tablets were taken as control drug. To detect the expression levels of α-SMA in liver by using immunohistochemistry and to detect the expression levels of HGF and c-Met mRNA by using real-time quantitative PCR. Results： The results of immunohistochemistry showed that compared with the model group and the colchicine group， KXYA group could significantly reduce the expression of α-SMA， which is of statistical significance (P<0.01， P<0.05). The results of real-time quantitative PCR showed that compared to the relative expression of HGF mRNA， KXYA group were significantly different with model group and colchicine group(P<0.05). Kangxian Yi-ai formula can significantly increase the expression level of HGF mRNA in rat liver precancerous lesions. The expression of c-Met mRNA were low in each group of DEN-induce liver precancerous lesions， and there is no statistical significance(P>0.05). Conclusion： Kangxian Yi-ai formula can increase the expression of HGF mRNA levels of hepatic lesions in rats， which may be the one of its prevention and treatment mechanism. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
