| 引用本文:陈红英1，李贤玉2，王文杰1，彭吉霞2.参麦对大鼠急性心肌缺血血管内皮生长因子表达的影响[J].世界中医药,2015,10(09):. |
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| 参麦对大鼠急性心肌缺血血管内皮生长因子表达的影响 |
| Effect of Shenmai on the Expressions of Myocardial Vascular Endothelial Growth Factors in Rats with Acute Myocardial Ischemia |
| 投稿时间：2014-10-13 |
| DOI：10.3969/j.issn.1673-7202.2015.09.025 |
| 中文关键词: 大鼠急性心肌缺血 参麦 血管内皮生长因子 缺氧诱导因子-1 受体胎肝激酶-1 |
| English Keywords:Acute myocardial ischemia of rats Shenmai injection Vascular endothelial growth factor Hypoxia inducible factor-1 receptor in fetal liver kinase-1 |
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| 中文摘要: |
| 目的：观察参麦对大鼠急性心肌缺血血管内皮生长因子表达的影响，探讨其作用机制。方法：大鼠40只，采用结扎左侧冠状动脉前降支30 min后松解扎扣再灌注60 min，反复3次以造成心肌缺血再灌注损伤模型，并随机分为对照组、模型组、美托洛尔组及参麦小大剂量组。五组大鼠均经腹腔注射给药，于治疗前及30 d后分别采用反转录-聚合酶链反应(RT-PCR)和免疫组织化学检测心肌VEGF和缺氧诱导因子-1a(HIF-1a)HIF-la表达情况、受体胎肝激酶1(FLK-1)，籍此考察参麦对大鼠急性心肌缺血血管内皮生长因子表达的影响。结果：参麦明显增高VEGF蛋白质及mRNA、HIF-1a、FLK-1的表达，缩小心肌缺血面积，与模型组比较（P<0.05），差异均有统计学意义，参麦小、大剂量组间差异无统计学意义。结论：参麦可减轻大鼠急性心肌缺血缺氧性损伤和血管内皮炎症反应程度，其作用机制可能是其促进大鼠缺血心肌血管内皮再生有关，对改善再灌注损伤血管内皮功能紊乱有一定的防治作用。 |
| English Summary: |
| To observe the effect of Shenmai on expressions of myocardial vascular endothelial growth factors in rats with acute myocardial ischemia， and explore its mechanism. Methods： 40 rats received ligation of left anterior descending coronary artery. 30 minutes later， release buckles reperfusion for 60 min to cause myocardial ischemia reperfusion injury model. The rats were then randomly divided into control group， model group， positive drug metoprolol group and Shenmai small dose， and Shenmai large dose group. Five groups of rats were treated with intraperitoneal injection， before treatment and 30 days respectively by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical detection of myocardial VEGF and hypoxia inducible factor -1a (HIF-1a) HIF-la expression， receptor in fetal liver kinase 1 (FLK-1)， the study of acute effect of nationality myocardial ischemic myocardium in rats of vascular endothelial growth factor expression. Results： Shenmai injection significantly increased the expression of VEGF protein and mRNA， HIF-1a， FLK-1， reduce the myocardial ischemic area， compared with the model group (P<0.05)， the difference was statistically significant， no significant difference in small， large dose Shenmai group. Conclusion： Shenmai injection can reduce the rat model of acute myocardial ischemia and hypoxia injury and vascular endothelial inflammatory reaction. Its mechanism may be related to the vascular endothelial regeneration in rats with myocardial ischemia reperfusion injury. It helps to improve the prevention of vascular endothelial dysfunction. |
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