To observe the effect of Shenmai on expressions of myocardial vascular endothelial growth factors in rats with acute myocardial ischemia， and explore its mechanism. Methods： 40 rats received ligation of left anterior descending coronary artery. 30 minutes later， release buckles reperfusion for 60 min to cause myocardial ischemia reperfusion injury model. The rats were then randomly divided into control group， model group， positive drug metoprolol group and Shenmai small dose， and Shenmai large dose group. Five groups of rats were treated with intraperitoneal injection， before treatment and 30 days respectively by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical detection of myocardial VEGF and hypoxia inducible factor -1a (HIF-1a) HIF-la expression， receptor in fetal liver kinase 1 (FLK-1)， the study of acute effect of nationality myocardial ischemic myocardium in rats of vascular endothelial growth factor expression. Results： Shenmai injection significantly increased the expression of VEGF protein and mRNA， HIF-1a， FLK-1， reduce the myocardial ischemic area， compared with the model group (P<0.05)， the difference was statistically significant， no significant difference in small， large dose Shenmai group. Conclusion： Shenmai injection can reduce the rat model of acute myocardial ischemia and hypoxia injury and vascular endothelial inflammatory reaction. Its mechanism may be related to the vascular endothelial regeneration in rats with myocardial ischemia reperfusion injury. It helps to improve the prevention of vascular endothelial dysfunction.