To explore the method of establishing animal model with premature ovarian failure due to chemotherapy damage by using cisplatin clinical medicine. Methods:According to the observation of vaginal exfoliated cells, we divided 60 female SD rats with normal estrous cycle into normal group, low dose group and high dose group randomly. Low dose group were given 4 mg·kg-1 intraperitoneal injection of Cisplatin and high dose group 6 mg·kg-1 respectively. The injection was given once a week and lasting for two weeks. The general conditions, weight, estrous cycle of the rats were observed every day. The serum levels of E2, FSH, LH and progesterone levels were detected after ovarian. The pathological and morphological changes were also observed under optical microscope. Results: Weight loss, estrous cycle disorder, anestrus prolongation were observed. Compared with normal status, the E2 levels of both high and low dose group decreased in different degree, while FSH, LH levels elevated.(P<0.05). No significant difference of progesterone was observed in both groups(P>0.05). Follicle also decreased. Conclusion: The declination of ovarian function of rats with cisplatin chemotherapy drugs is obvious, follicle development and reproductive capacity are impaired, which provides a simple, high success rated, and reliable method for animal model with premature ovarian failure.