| To investigate the mechanism of Ganhuangcha Cream in treating Periodontitis. Methods: The Kunming mice were divided into four groups by random number table, each group has 10 cases. The group given none-treatment was regarded as control group, the rest three groups were treated with 20%, 40%, 80% Ganhuangcha Cream respectively as Low, Medium, High concentration group. After the treatment, the positive expressions of gingival index(GI), serum IgG, periodontium BMP2, IGF1, bFGF were compared among groups. Results: Compared with the control group, GI, serum IgG levels of Low, Medium, High concentration group significantly increased, the positive expressions of IGF1, BMP2, bFGF were significantly lower, the difference was statistically significant(P<0.05). After treated for 1 to 2h, GI, serum IgG of High concentration group were lowest, Low concentration group were highest. After treated for 4 to 6h, Medium concentration group were lowest; Low concentration group were highest, the difference was statistically significant (P<0.05). GI, serum IgG of Low, High concentration group increased gradually as the treating time prolonged, while the Medium concentration group decreased. The positive expressions of BMP2, IGF1 and bFGF were highest in Medium concentration group, followed by High concentration group, and then the Low concentration group, the difference was statistically significant (P<0.05). Conclusion: Ganhuangcha Cream can treat periodontitis effectively by anti-inflammation and and repairing periodontium, which is related to concentration of the drug.The treatment effect is the best when treated by 40% Ganhuangcha Cream for 4h. It provides guidance for the clinical treatment of periodontitis. |
