| 引用本文:李尚蓉1,2，张静娴2，姚帅2，果德安2，吴婉莹2.珍珠和珍珠母的微量元素测定及其比较分析[J].世界中医药,2015,10(10):. |
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| 珍珠和珍珠母的微量元素测定及其比较分析 |
| Comparative Study of Trace Elements in Pearls and Nacres |
| 投稿时间：2015-02-04 |
| DOI：10.3969/j.issn.1673-7202.2015.10.037 |
| 中文关键词: 珍珠 珍珠母 微量元素 PCA OPLS-DA |
| English Keywords:Pearl Nacre Trace element PCA OPLS-DA |
| 基金项目:国家高技术研究发展计划(863计划)(编号:2013AA093002)——“海洋传统药源生物(中药)资源开发利用” |
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| 中文摘要: |
| 目的:全面测定珍珠和珍珠母中的微量元素,对其在2味药材中的分布情况进行比较分析,找出差异性。方法:采用电感耦合等离子体质谱(ICP-MS)法和电感耦合等离子体发射光谱(ICP-OES)法对8批珍珠和9批珍珠母中24种微量元素进行测定,并运用SIMCA-P+13.0软件,采用主成分分析法(Principal Component Analysis,PCA)和正交偏最小二乘-判别分析法(Orthogonal Partial Least Squares-discriminant Analysis,OPLS-DA)对测定结果进行统计分析。结果:在24种微量元素中,珍珠和珍珠母中共检测出了16种,其中Ca含量最高,其次是Na,Mn,Sr,Mg,Fe。PCA得分图显示2味药材可以明显区分开,OPLS-DA结果表明二者差异较大的微量元素为Na。结论:本研究全面阐明了珍珠和珍珠母中微量元素和二者的差异性,为珍珠粉与贝壳粉的区分提供了依据,同时为二者的质量控制提供了参考。 |
| English Summary: |
| To comprehensively compare and detect the trace elements of pearls and nacres in the purpose of finding their differences. Methods:ICP-MS and ICP-OES were used to determine the 24 kinds of trace elements in 8 batches of pearls and 9 batches of nacres. The results were taken to principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) by using SIMCA-P+ 13.0 software to analyze the distribution of the trace elements in the two drugs and also to detect the discriminatory constituents. Results:Among the 24 kinds of trace elements, 16 were detected in both pearls and nacres. It was found that Ca was the most abundant one, which was followed by Na, Mn, Sr, Mg, and Fe. The scatter plot from PCA indicated that pearls and nacres can be easily discriminated, as is proved by the result from OPLS-DA, which showed that the most discriminatory element was Na. Conclusion:The study described the distribution and differences in the trace elements between pearls and nacres, and provided important information for the differentiation of pearl powder and conch powder. This also can be taken as the reference to the quality control of the two drugs. |
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