To establish the quality standard for Gusongling Granules. Methods: TLC was performed to identify the qualification of Epimedii Folium(Yinyanghuo), Drynarial Rhizoma(Gusuibu), Psoraleae Fructus(Buguzhi), Rehmanniae Radix Praeparata(Shudihuang), Astragali Radix(Huangqi), Angelicae Sinensis Radix(Danggui), Eucommiae Cortex(Yanduzhong), Testudinis Carapax Et Plastrum(Cuguijia). Besides, a HPLC method was established for the determination of icariine, naringin, acteoside, and astragaloside. Results: The spots on TLC plates were clear with specificity. Icariin showed a good linear relationship at a range between 0.0315 and 1.7120 μg, r=0.9997, the average recovery was 100.23%, and RSD was 2.23%. Naringin showed a good linear relationship at a range between 0.0568 and 1.8175 μg, r=0.9996, the average recovery was 99.97%, and RSD was 1.22%. Acteoside showed a good linear relationship at a range between 0.0608 and 1.9456 μg, r=0.9998, the average recovery was 99.94%, and RSD was 1.24%. Astragaloside showed a good linear relationship at a range between 0.4676 and 11.2200 μg, r=0.9998, the average recovery was 99.84%, and RSD was 3.35%. Conclusion: This method can be accurate and reliable in qualitative and quantitative detection, and can control the drug quality effectively.