To explore the efficacy honey-fried Radix asteris decoction (HFRAD) of different concentration on cell cycle and proliferation of colorectal cancer LOVO cells.Methods:The changes of cellular morphology of LOVO cells treated with different concentration HFRAD were observed by optical microscopy. MTT colorimetric assay was applied to observe proliferation of LOVO cells; flow cytometry was used to analyze cell cycle of LOVO cells. Results:MTT results indicated that proliferation of LOVO cells treated with low concentration HFRAD（≤20mg·mL-1）has been obviously promoted, and the cell proliferation rate could be up to 99.7%. Moreover, normal cellular morphology and membrane structure were observed by optical microscopy. Compared with the low concentration groups, it was found that cell proliferation of LOVO cells treated with high concentration HFRAD (>20mg·mL-1) was obviously inhibited, the cell inhibitor rate can be up to above 80.34%. Optical microscopy results indicated that LOVO cells treated with high concentration HFRAD had lowest cell viability and the number of normal morphology cells can be significantly reduced. Flow cytometry results showed that the proportions of G1, S and G2 phase cells vary from different concentration HFARD and were significantly different from the control group. The proportion of S phase LOVO cells increased when treated with 10 and 20mg·mL-1 HFRAD. However, it was also found that the proportion of S phase LOVO cells decreased and the proportion of G2 phase increased with 30mg·mL-1 HFRAD. Conclusion:Different concentrate HFRAD has distinct efficacy on cell proliferation and cell cycle of LOVO cells. Cell proliferation of LOVO cells can be significantly promoted in concentration of HFRAD（≤20mg·mL-1）. However, high concentration HFRAD(>20mg·mL-1) can inhibit cell proliferation. Moreover, inhibition of G2 phase might be due to high concentration HFRAD. Our findings can provide experimental evidences for HFRAD in clinical therapy of Colorectal cancer.