| 引用本文:杨映映，秦甜甜，钱树树，彭林，刘莉嘉，白松棉，王如峰，蒋燕，胡秀华.不同浓度蜜炙紫菀水煎剂对大肠癌LOVO细胞增殖和周期的影响[J].世界中医药,2015,10(11):. |
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| 不同浓度蜜炙紫菀水煎剂对大肠癌LOVO细胞增殖和周期的影响 |
| Effects of Honey-fried Radix Asteris Decoction（HFRAD） on Cell Proliferation and Cell Cycle of Colorectal Cancer LOVO Cells |
| 投稿时间：2015-01-16 |
| DOI：10.3969/j.issn.1673-7202.2015.11.032 |
| 中文关键词: 大肠癌LOVO细胞 蜜炙紫菀水煎剂 细胞增殖 细胞周期 |
| English Keywords:Colorectal cancer LOVO cells Honey-fried Radix asteris decoction (HFRAD) Cell proliferation Cell cycle |
| 基金项目:国家级大学生创新创业训练计划课题——基于“肺与大肠相表里”理论研究紫菀及其提取物对大肠癌细胞增殖和迁移的影响（编号：20131002602）；国家自然基金面上项目（编号：81274044） |
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| 中文摘要: |
| 目的：探讨不同浓度蜜炙紫菀水煎剂对大肠癌LOVO细胞增殖、周期的影响。方法：光学显微镜下观察蜜炙紫菀水煎剂对LOVO细胞形态的影响；利用MTT观察药物处理后对LOVO细胞的生长增殖情况的影响；采用流式细胞术检测蜜炙紫菀水煎剂对大肠癌LOVO细胞增殖周期的影响。结果：光学显微镜和MTT结果发现，低浓度的蜜炙紫菀水煎剂（≤20 mg·mL-1）对大肠癌LOVO细胞的增殖具有明显的促进作用，增殖率可达到99.7%；光学显微镜下可见细胞生长状态良好，细胞死亡少。高浓度的蜜炙紫菀水煎剂（＞20 mg·mL-1）对大肠癌LOVO细胞的增殖具有明显的抑制作用，抑制率达到80.34%以上；光学显微镜下可见细胞的形态改变，细胞大量死亡。流式细胞术结果显示，蜜炙紫菀水煎剂可以使大肠癌LOVO细胞周期各个时相（G1期、S期和G2期）的细胞所占百分数发生改变。10 mg·mL-1和20 mg·mL-1的蜜炙紫菀水煎剂使S期中细胞所占的百分数增加；30 mg·mL-1蜜炙紫菀水煎剂使S期细胞所占的百分数明显减少，G2期细胞百分数明显增加。结论：蜜炙紫菀水煎剂对大肠癌LOVO细胞增殖的作用是双向的，低浓度（≤20 mg·mL-1）的蜜炙紫菀水煎剂具有明显的促进LOVO细胞增殖作用；高浓度（＞20 mg·mL-1）的蜜炙紫菀水煎剂对大肠癌LOVO细胞的增殖具有明显的抑制作用，且可能是通过G2期阻滞来抑制大肠癌LOVO细胞增殖的，这为大肠癌的临床治疗提供了新的思路。 |
| English Summary: |
| To explore the efficacy honey-fried Radix asteris decoction (HFRAD) of different concentration on cell cycle and proliferation of colorectal cancer LOVO cells.Methods:The changes of cellular morphology of LOVO cells treated with different concentration HFRAD were observed by optical microscopy. MTT colorimetric assay was applied to observe proliferation of LOVO cells; flow cytometry was used to analyze cell cycle of LOVO cells. Results:MTT results indicated that proliferation of LOVO cells treated with low concentration HFRAD（≤20mg·mL-1）has been obviously promoted, and the cell proliferation rate could be up to 99.7%. Moreover, normal cellular morphology and membrane structure were observed by optical microscopy. Compared with the low concentration groups, it was found that cell proliferation of LOVO cells treated with high concentration HFRAD (>20mg·mL-1) was obviously inhibited, the cell inhibitor rate can be up to above 80.34%. Optical microscopy results indicated that LOVO cells treated with high concentration HFRAD had lowest cell viability and the number of normal morphology cells can be significantly reduced. Flow cytometry results showed that the proportions of G1, S and G2 phase cells vary from different concentration HFARD and were significantly different from the control group. The proportion of S phase LOVO cells increased when treated with 10 and 20mg·mL-1 HFRAD. However, it was also found that the proportion of S phase LOVO cells decreased and the proportion of G2 phase increased with 30mg·mL-1 HFRAD. Conclusion:Different concentrate HFRAD has distinct efficacy on cell proliferation and cell cycle of LOVO cells. Cell proliferation of LOVO cells can be significantly promoted in concentration of HFRAD（≤20mg·mL-1）. However, high concentration HFRAD(>20mg·mL-1) can inhibit cell proliferation. Moreover, inhibition of G2 phase might be due to high concentration HFRAD. Our findings can provide experimental evidences for HFRAD in clinical therapy of Colorectal cancer. |
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