Study on warm-heat natural herbs(adix Aconiti Lateralis Preparata，Rhizoma Zingiberis，Pricklyash Peel)energy metabolism and expression of liver protein in normal rats，and made warm-hot drug in property and flavor-effect-pharmacological effects difference sand similarities，to provide theoretical basis for the property and flavor of medicines scientific connotation of warm-heat natural herbs.Methods:Adopt Sprague Dawley rats，each group after continuous administration of 21 d，the rats were anesthetized via an intraperitoneal injection of 1% pentobarbital sodium(0.15 mL per 100 g body weight).Liver proteins are separated by differential centrifugation，BCA protein quantitation by enzyme linked immunosorbent assay(ELISA)，using iTRAQ technology combined with the 2D-LC-MS/MS analysis method identification of liver proteins.Results:Compared with the control group，warm-heat natural herbs have identified significantly altered 50 differential proteins，with 39 proteins significantly increased and 11 decreased in response.50 different proteins mainly distributed in 18 pathways，many pathways related to energy metabolism.Conclusion:Radix Aconiti Lateralis Preparata，Rhizoma Zingiberis and Pricklyash Peel such as warm-heat natural herbs have differences and similarities in proteomics.This commonality is reflected not only in the substance and energy metabolism，but also reflected in the pharmacological effects.Two aspects affect the bodys energy metabolism：increasing heat generation and increase the energy utilization.The results of the study for “Chinese medicine has the property(gas)and flavor，the characteristics of characteristics of property(gas)and flavor.Flavor of a drug was related with specific efficacy.Property of a drug affects the bodys energy metabolism and substance metabolism by different pass ways.Influencing therapeutic effects of drugs or lead to side-effect associated with biological effects.” Nature and flavor of medicines theory provides a scientific basis.At the same time as the evaluation of traditional Chinese medicine provides a new method for Cold and Hot natural properties.