Toestablished the split method of nature and flavor material foudition of Cortex Mori，analysis of Cortex Mori chemical characteristics of the split components by HPLC，validae the unoverlapping analysis of Chemical Constituents of Cortex Mori in chemometrics methods.Methods:Established Cortex Mori chemical composition of the split method by steam distillation，extraction，alcohol，macroporous adsorption resin technology.With the combined applications of steam distillation，water extraction and alcohol precipitation，liquid-liquid extraction and column chromatography over macroporous resin，thesplitted-fractions method of the chemical constituents of poriacocos was established; Unoverlapping Analysis of Chemical Constituents of Cortex Mori by combining the HPLC-PDA and HPLC-ELSD in ChemPatternclustering analysis and principal component analysis.Results:We splitted the material basis of decoction of Cortex Mori for 5 groups，each group compositions substantially free of chemical components intersect.The process is stable and controllable by 10 batch in similarity evaluation.Conclusion:The split technology of nature and flavor material foundation of Cortex Mori is stable and practical，Indicated that Cortex Mori resolvability constituent holo-composition which was detached uncross as each one could be well separated under no supervision mode.