| 引用本文:迟莉丽1，宋钦兰1，程艳1，孙大娟1，闫华1，梁峻尉1，王帅1，袁浩2.中药安肠愈疡汤对溃疡性结肠炎大鼠结肠组织中IL 6、IL 13含量及NF κB表达的影响[J].世界中医药,2016,(01):. |
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| 中药安肠愈疡汤对溃疡性结肠炎大鼠结肠组织中IL 6、IL 13含量及NF κB表达的影响 |
| Effect of Anchang Jieyu Decoction on the Expression of IL 6, IL 13 and NF κB in Ulcerative Colitis Mice |
| 投稿时间：2015-07-12 |
| DOI：10.3969/j.issn.1673-7202.2016.01.030 |
| 中文关键词: 溃疡性结肠炎 中药安肠愈疡汤 IL 6 IL 13 NF κB 实验研究 |
| English Keywords:Ulcerative colitis Anchang Jieyu decoction IL 6 IL 13 NF κB Experiment |
| 基金项目:山东省自然科学基金（编号：ZR2012HL11）——“溃疡性结肠炎大鼠结肠组织NF κB、TLR 4表达与其损伤关系及中药复方干预的研究” |
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| 中文摘要: |
| 目的：观察中药复方安肠愈疡汤对溃疡性结肠炎（Ulcerative Colitis，UC）大鼠结肠组织中IL 6、IL 13含量及NF κB表达的影响，以探讨其作用机制。方法：将90只Wistar大鼠随机分为6组（空白对照组，模型对照组，安肠愈疡汤低、中、高剂量组和美沙拉嗪组）。除空白组外，其他各组均应用三硝基苯磺酸(TNBS)制备UC大鼠模型，造模成功后，按照组别分别给予不同剂量的实验药物，连续给药3周后，进行结肠组织肉眼形态评分，同时采用ELISA法检测结肠组织中IL 6、IL 13含量，免疫组化法检测NF κB蛋白表达。结果：安肠愈疡汤中、高剂量组及美沙拉嗪组大鼠结肠黏膜均有不同程度的炎症修复、溃疡愈合（P<0.01或P<0.05），其中高剂量组及美沙拉嗪组明显优于中、低剂量组，但2组比较无统计学意义（P>0.05）；在IL 6方面，安肠愈疡汤低、中、高剂量组及美沙拉嗪组的含量均不同程度降低（P<0.05或P<0.01），其中，高剂量组及美沙拉嗪组明显优于中、低剂量组(P<0.01)，但2组之间无统计学意义（P>0.05）；在IL 13方面，安肠愈疡汤中、高剂量组及美沙拉嗪组的含量均不同程度升高（P<0.01或P<0.05），尤以高剂量组效果最好，与美沙拉嗪组比较有差异有统计学意义(P<0.05)；在NF κB表达方面，安肠愈疡汤中、高剂量组及美沙拉嗪组均有不同程度降低（P<0.05或P<0.01），其中高剂量组效果最好，但与美沙拉嗪组相比无统计学意义（P>0.05）。结论：安肠愈疡汤具有较好的抗炎和促进溃疡修复的作用，可能是通过下调致炎因子IL 6，上调抗炎因子IL 13，同时降低NF κB表达，从而起到治疗UC的作用。 |
| English Summary: |
| To explore the mechanism of Anchang Jieyu decoctiona by detecting its effect on the expression of IL 6, IL 13 and NF kB in rats with ulcerative colitis(UC).Methods:Ninety Wistar rats were randomly divided into six groups(blank control group, model control group, Anchang Jieyu decoctiona low, medium, high dose group and Solfasalazine(5 ASA) group). Except for the blank group, the other groups were given trinitrobenzene sulfonic acid(TNBS) to produce rat model of UC. Different groups were given different dose of drugs respectively. After 3 weeks administration, colon tissue of the eye morphology scores, IL 6, IL 13 content, and NF κB protein expression were detected.Results:Rats in Anchang Jieyu decoctiona low, medium, high dose group and 5 ASA group showed varying degrees of improvement and ulcer healing(P<0.01 orP<0.05), of which the high dose group and the 5 ASA were superior to others, but comparison between the two groups showed no statistical significance(P>0.05); In level of IL 6, TCM low, medium and high dose group and the 5 ASA group were differently lower(P<0.05 orP<0.01). Among them, the high dose group and the 5 ASA were superior to middle and low dose group (P<0.01), but no statistical significance had showed between the two groups (P>0.05); In level of IL 13, TCM high dose group and 5 ASA group were differently increased (P<0.01 orP<0.05), especially in high dose group. Besides, compared with 5 ASA group, there was significant differences(P<0.05); In level of NF kB, TCM medium, high dose group and 5 ASA group have declined (P<0.05 orP<0.01), in which the effect of high dose group performed the best. While compared with 5 ASA group, there were no statistical significance (P>0.05).Conclusion:Anchang Jieyu decoction performs better in anti inflammation and ulcer repair promotion. This may through down regulating pro inflammatory cytokines IL 6, up regulating anti inflammatory cytokines IL 13, and inhibiting the expression of NF κB, which contribute a lot to the treatment of UC. |
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