To explore different active chemical components in Radix paeoniae alba (stir baked with vinegar), Glyycrrhizae radix (stir baked with honey) and their drug pairs. Methods:Water boiled Radix paeoniae alba, Glyycrrhizae radix respectively and use HCPL method to test their solution. Results:The four active constitutes (Oxypaeoniflorin, Albiflorin, Paeoniflorin, Benzoylpaeoniflorin) were [1.07+0.12, 0.36+0.02, 2.00+0.21, 0.37+0.03] in Radix paeoniae alba(vinegar baked); [1.89+0.11, 0.86+0.05, 3.25+0.13, 0.52+0.03] in Radix Paeoniae alba(vinegar baked) Glyycrrhizae radix and [1.90+0.09,0.87+0.04,3.30+0.24,0.52+0.01] in Radix paeniae alba Glyycrrhizae radix(honey fried); The five active constituents(Glycyrrhizic acid, Liquiritin, Liquiritigenin, Isoliquiritin, Isoliquiritigenin) were[6.53+0.25,2.17+0.33,0.52+0.21,0.48+0.23,0.09+0.03,9.79+0.65] in Glyycrrhizae radix; [5.13+0.32,2.11+0.21,0.47+0.12,0.57+0.22,0.12+0.02,8.40+0.75] in honey fried Glyycrrhizae radix focal Radix paeoniae alba; [6.47+0.33,2.67+0.23,0.56+0.23,0.60+0.23, 0.12+0.01, 10.42+0.78] in honey fried Glyycrrhizae radix parched Radix paeoniae alba and [8.76+0.35,3.10+0.43,0.79+0.32,0.76+0.34,0.15+0.05,13.56+0.99] in honey fried Glyycrrhizae radix vigar baked Radix paeoniae alba. Conclusion:Different combination of herbs changed the physical and chemical nature of the single herb, and thus changed its active components. The crucial point in Chinese medicine decoction lies in different components and their proportion of active components. These could affect the curative effect of decoction. It is of great significance to discriminate different active constitutes in drug pairs as it has distinct clinical effect. |