引用本文:李亚勤1,于波1,李铁1,赵宗江2,杨美娟2,王富春1.针刺单穴及腧穴配伍对糖尿病胃轻瘫大鼠胃窦Cajal间质细胞影响[J].世界中医药,2016,(02):. |
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针刺单穴及腧穴配伍对糖尿病胃轻瘫大鼠胃窦Cajal间质细胞影响 |
Effect of Acupuncture at Single Acupoint and Combined Acupoints on Antral Interstitial Cells of Cajal in Diabetic Gastroparesis Model Rats |
投稿时间:2016-01-12 |
DOI:10.3969/j.issn.1673-7202.2016.02.007 |
中文关键词: 糖尿病胃轻瘫 针刺 腧穴配伍 Cajal间质细胞 |
English Keywords:Diabetic gastroparesis Acupuncture Acupoint combination Interstitial cells of Cajal |
基金项目:国家重点基础研究发展计划(“973”计划)项目(编号:2014CB543100);吉林省卫生厅项目(编号:2014Q048) |
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中文摘要: |
目的:探讨针刺单穴及腧穴配伍对糖尿病胃轻瘫(Diabetic gastroparesis,DGP)大鼠胃窦Cajal间质细胞(Interstitial Cells of Cajal,ICC)的影响。方法:选取雄性SD大鼠50只,随机分为正常组、模型组、多潘立酮组、足三里组、中脘+足三里组,每组10只。以链脲佐菌素(STZ)腹腔注射诱导DGP大鼠模型。采用免疫组化技术观察各组大鼠ICC含量的改变。结果:与正常组相比,模型组大鼠胃窦组织ICC含量明显减少(P<0.01);与模型组比较,多潘立酮组(P<0.01)、足三里组(P<0.05)、中脘+足三里组(P<0.01)大鼠胃窦ICC含量明显提高,且中脘+足三里组优于足三里组(P<0.05)。结论:针刺促进DGP大鼠胃窦ICC含量恢复,腧穴配伍组优于单穴组。 |
English Summary: |
To explore the effects of acupuncture at single acupoint and combined acupoints on antral interstitial cells of Cajal (ICC) in diabetic gastroparesis model rats. Methods: 50 SD male rats were randomly divided into 5 groups with 10 rats in each, namely normal group, diabetic gastroparesis model rats group (the model group), domperidone group,acupuncture at ST36 group and acupuncture at ST36+CV12 group. The rats were intraperitoneally injected with steptozotocin to set diabetic gastroparesis model. Treatments for each group lasted for 5 weeks. Numbers of ICC were detected by immunohistochemical methods. Results: The number of ICC in the model group were significantly reduced (P<0.01), compared with normal group, while numbers of ICC in domperidone group (P<0.01), acupuncture at ST36 group (P<0.05) and ST36+CV12 group (P<0.01) were improved significantly, compared with the model group. Numbers of ICC in the acupuncture at ST36+CV12 group was increased remarkably superior to the acupuncture at ST36 group (P<0.05). Conclusion:Acupuncture could improve the numbers of antral interstitial cells of Cajal in diabetic gastroparesis model rats, and acupuncture at combined acupoints was better than that of the single acupoint. |
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