To study the immediate effect of Indonesian herbal solution Jamkho upon total blood cholesterol level. Methods: Subjects were clients/patients of a traditional medicine clinic in Jakarta who had agreed to be tested their random blood cholesterol levels pre and one hour post ingestion of two spoonful of herbal solution Jamkho. The pre and post ingestion data were analyzed using paired t-test. Results: There were 41 subjects included, consisting of 21 female and 20 male, with an average of （45.66±14.03） years old. Their average random blood total cholesterol level before given Jamkho was （232. 98±37.02） mg/dL, and after given Jamkho was （194.12±34.85） mg/dL. The difference was highly significant (P<0.01), while subgroup with initial blood total cholesterol levels below 200 mg/dL did not show significant changes of blood total cholesterol level (P>0.05) after ingestion of Jamkho. Conclusion: The herbal solution Jamkho could effectively lower random blood total cholesterol levels very significantly (P<0.01) upon subjects studied, especially those with initial total blood cholesterol levels 200 mg/dL and above. But upon subjects with initial blood total cholesterol levels below 200 mg/dL, Jamkho did not change the levels significantly (P>0.05).