| 引用本文:张淑静，吴莹，齐晓宇，葛东宇，李根茂，玄子男，王谦，王旭丹，李姝玉.小鼠流感病毒性肺炎模型中肠道的病变及在中医理论研究中的应用[J].世界中医药,2016,(02):. |
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| 小鼠流感病毒性肺炎模型中肠道的病变及在中医理论研究中的应用 |
| Pathological Damage of Intestine in the Viral Pneumonia Mouse Model and its Application in the Theoretical Research of Traditional Chinese Medicine |
| 投稿时间：2015-08-20 |
| DOI：10.3969/j.issn.1673-7202.2016.02.028 |
| 中文关键词: 流感病毒 肺炎 肠道免疫病理 肺与大肠相表里 |
| English Keywords:Influenza virus Viral pneumonia mouse model Intestinal munological pathology the lung and the large intestine being interior-exteriorly related |
| 基金项目:国家自然科学基金面上项目(编号:81473520) |
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| 中文摘要: |
| 目的:建立小鼠流感性肺炎模型,明确呼吸道流感病毒感染过程中是否存在特异性的肠道病理损伤,并探讨了其在“肺与大肠相表里”研究中的应用。方法:BALB/c小鼠48只随机分为正常组、病毒组。病毒组给予50 μL、50LD50病毒液滴鼻建立流感病毒感染的小鼠肺炎模型,感染后第3、5、6天分别取材,HE染色检测肺脏、大肠、小肠、心脏、肝脏、肾脏组织病理结果,血生化检测血清谷草转氨酶(AST)、谷丙转氨酶(ALT)和尿素氮(BUN)水平。结果:流感病毒感染后,病毒组小鼠肺组织出现明显的炎性病变外,随时间延长肺组织充血水肿出血加重;小肠黏膜腺体排列紊乱,绒毛粗大、部分脱落,黏膜固有层炎性细胞浸润;大肠黏膜固有层、肌层大量炎性细胞浸润,腺体结构消失、脱落;而心脏、肝脏、肾脏无明显病理改变;病毒组小鼠血清AST、ALT、BUN较正常组相比,差异无统计学意义。结论:小鼠流感病毒性肺炎模型中存在小肠、大肠特异性免疫病理损伤,是研究中医“肺与大肠相表里”理论及相关临床治法的理想模型。 |
| English Summary: |
| To establish viral pneumonia mouse models infected by influenza virus, and observe whether there is a specific intestinal pathological damage in the process of respiratory influenza virus infection, then discuss its application on the research of the theory “the lung and the large intestine being interior-exteriorly related” in Traditional Chinese Medicine. Methods: A total of 48 BALB/c mice were randomly divided into the normal group and the virus group. Influenza virus 50 μL 50 LD50 and mouse lung-adapted strain, was intranasally inoculated to the virus group. On the 3, 5 and 6 day of infection, HE staining was used to detect the pathologic changes /in lungs, large intestines, small intestines, hearts, livers and kidneys. In addition, blood biochemistry was used to detect the levels of aspertate aminotransferase(AST), alanine aminotransferase(ALT), urea nitrogen(BUN) in serum. Results: After infection, the lungs of the mice in the virus group showed obvious inflammatory pathological changes, and hyperemia edema, hemorrhage in the lung tissues aggravated with time; the arrangement of mucosal glands in the small intestines was disordered with the villus swelling and partial detachment, and the mucosa lamina propria was infiltrated with lots of inflammatory cells; the large intestine lamina propria and the muscular layer has been infiltrated with large number of inflammatory cells and the muscle layer glandular structures disappeared. However, the heart, liver and kidney had no obvious pathological changes, and the AST, ALT, BUN levels in the virus group showed no significant difference. Conclusion: The viral pneumonia mouse model infected with influenza virus exists specific immunologically pathological damage in the small intestine and the large intestine. It serves as an ideal model to study the Traditional Chinese Medicine theory “the Lung and the Large Intestine being interior-exteriorly related” and the related clinic treatment. |
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