世界中医药

作者	单位
武蕊娟1,2，谭镭2，詹，雁2，阮佳2，徐超2	1 成都中医药大学，成都，610075； 2 四川省中医药科学院，成都，610041

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中文摘要:

目的：从药用植物马甲子叶醇提物中分离筛选具有抗肿瘤作用的活性组分。方法：采用石油醚、乙酸乙酯、正丁醇萃取马甲子叶醇提物，并将这三个萃取部位进行体外抗肿瘤实验，再把筛选得到的具有较好抗肿瘤活性的部位进行硅胶柱层析分离，并采用MTT法对分离得到的组分进行体外抗肿瘤活性比较。结果：通过体外抗HepG-2细胞和HeLa细胞实验表明，乙酸乙酯萃取部位具有较好抗肿瘤活性。通过硅胶柱分离，获得5个组分，通过MTT实验发现这5个组分中，石油醚：乙酸乙酯为1∶1和1∶2洗脱所得到的两个组分具有明显的体外抗肿瘤活性，其中石油醚：乙酸乙酯为1∶2的洗脱具有更好的杀伤作用，对HepG-2细胞和HeLa细胞的IC50分别为45.79，13.16 μg/mL。结论：从马甲子叶醇提物乙酸乙酯部位中分离得到2种具有抗肿瘤作用的活性组分，为进一步分离抗肿瘤活性成分提供一定的指导作用。

English Summary:

To separate and select anti-tumor activity part from the leaves of Paliurus ramosissimus. Methods: Extracts from the leaves of Paliurus ramosissimus were extracted with petroleum ether, ethyl acetate and n-butyl alcohol respectively. In vitro anti-tumor experiments were performed at the three extract sites. The optimal site with best anti-tumor activity then was screened by silica gel column chromatography, and MTT assay was used for the comparison of components obtained for antitumor activity. Results: In vitro anti HepG-2 cells and HeLa cell experiments showed that ethyl acetate fraction extracts had good anti-tumor activity. Separated by a silica gel column, five components were collected by MTT assay, which found that when the ratio of petroleum ether: ethyl acetate were 1∶1 and 1∶2, both eluted fraction group had in vitro anti-tumor activity and when the ration of the elution of petroleum ether to ethyl acetate was 1∶2 the anti-tumor function was better. IC50 of HepG-2 cells and HeLa cells were 45.79 and 13.16 μg·mL-1 respectively. Conclusion: Two kinds of active ingredients with anti-tumor effect were separated from the leaves of Paliurus ramosissimus by ethyl alcohol, which may provide guidance for the further separation of anti-tumor active ingredient.

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