世界中医药

作者	单位
陈伟韬1,2，梁之桃2，周立敏2，周劲松3，赵中振2	1 广东省中医药工程技术研究院，广东广州，510095； 2 香港浸会大学，香港； 3 广州中医药大学，广东广州，510405

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中文摘要:

目的：建立布渣叶超高效液相指纹图谱，为快速有效对布渣叶进行质量评价，改进其质量控制方法提供理论依据。方法：采用Waters Acquity BEH UPLC C18色谱柱(2.1 mm×100 mm，1.7 μm)，以乙腈-0.1%甲酸水溶液作为流动相梯度洗脱，检测波长为278 nm，流速为0.3 mL/min，柱温为20 ℃；对建立的布渣叶指纹谱图进行相似度计算，并对数据进行主成分分析。结果：方法学考察结果良好，确立了11个共有峰，布渣叶指纹图谱的相似度明显高于伪品；主成分分析结果显示布渣叶密集分布在一个区域内，伪品分布在该区域外。结论：该方法稳定可靠，能对布渣叶药材作出真伪优劣评价，为建立布渣叶超高效液相指纹图谱提供理论依据。

English Summary:

To establish the chemical fingerprint of Microctis Folium by ultra performance liquid chromatography(UPLC).Methods:The separation was developed on ACQUITY UPLC BEH C18 column(2.1 mm×100 mm，1.7 μm)by gradient elution with acetonitrile-water containing 0.1% formic acid as mobile phase at a flow rate of 0.3 mL/min，the detection wavelength at 278 nm and column temperature at 20 ℃.The fingerprint was evaluated by similarity assay and principal component analysis(PCA).Results:Eleven characteristic peaks were established in the fingerprint，and two peaks were identified.The similarities of Microctis Folium was calculated and used for differentiating its adulterant.PCA showed that Microctis Folium and its adulterant were distinctly separated by the three most important principal components.Conclusion:The method is stable and reliable which could be applied to the authentication and quality control of Microctis folium.

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