| 引用本文:佟彤1，刘红旭1，尚菊菊1，杨志海2，邢文龙1，李享1.参元丹对缺血再灌注心肌细胞内质网应激相关因子GRP78、CHOP表达的影响[J].世界中医药,2016,(03):. |
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| 参元丹对缺血再灌注心肌细胞内质网应激相关因子GRP78、CHOP表达的影响 |
| Effects of Shen Yuan Dan on Grp78 and CHOP Expression in Ischemia-reperfusion Myocardium Cell |
| 投稿时间：2016-02-24 |
| DOI：10.3969/j.issn.1673-7202.2016.03.009 |
| 中文关键词: 参元丹 内质网应激 缺血再灌注损伤 |
| English Keywords:SYD Endoplasmic reticulum stress Ischemia-reperfusion injury |
| 基金项目:北京市科学技术委员会十病十药课题（编号：Z141100002214010）；北京市教委科技发展计划面上项目（编号：KM201310025027）；国家自然科学基金项目（编号：81273741）；首都中医药研究专项（编号：13ZY12） |
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| 中文摘要: |
| 目的：观察参元丹对缺血再灌注心肌细胞内质网应激相关因子Grp78、CHOP表达的影响。方法：离体培养新出生1～3 d大鼠心肌细胞，并分为正常组、模型组、参元丹组及空白血清组。应用三气培养箱制造缺血（2 h）/再灌注（4 h）模型。采用RT-PCR法测定内质网应激相关因子GRP-78、CHOP因子的表达。结果：模型组细胞内Grp78、CHOP表达均明显上升（P<0.01）。与模型组比较10%参元丹组中，Grp78（P<0.01）及CHOP（P<0.05）均有明显下降，10%空白血清组Grp78、CHOP较模型组无明显变化。结论：参元丹可以通过减轻内质网应激减少心肌细胞的凋亡，保护心肌细胞。 |
| English Summary: |
| To observe the effect of Shen Yuan Dan(SYD) on Grp78 and CHOP expression in ischemia-reperfusion myocardium cells. Methods: To culture cardiomyocytes from newborn rats, and then divided them into normal group, model group, SYD group and blank serum group. Ischemia (2 h) / reperfusion (4 h) model was established by tri-gas incubator. Real-time polymerase chain reaction (PCR) were used to detect the Grp78 and CHOP gene expression levels. Results: The Grp78, CHOP expression levels in model group were significantly increased (P<0.01). Compared with model group, Grp78 and CHOP were significantly decreased in 10% SYD group (P<0.05). Levels of Grp78 and CHOP has no obvious change between 10% blank serum group and model group. Conclusion: SYD can reduce endoplasmic reticulum stress in myocardial cell apoptosis and protect myocardial cells. |
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