世界中医药

目的：考察制何首乌水提物对大鼠肝细胞色素P450酶亚型CYP1A2的酶活性及mRNA表达的影响。方法：水回流提取得制何首乌水提物，HPLC确定主要成分并测定其含量。大鼠随机分为空白对照组、制何首乌水提物组（2 g/kg和20 g/kg），给药组每天按10 mL/kg量灌胃给予制何首乌水提物，连续7 d。取肝分离肝微粒体，HPLC测定肝微粒体对CYP1A2酶的底物非那西丁的代谢消除率，考察肝微粒体CYP1A2酶活性；实时荧光定量PCR技术检测肝组织中CYP1A2基因mRNA的表达。结果：与空白对照组相比，制何首乌水提物20 g/kg组大鼠肝CYP1A2酶活性和mRNA的表达均明显降低（CYP1A2酶活性，P<0.01；CYP1A2的mRNA表达，P<0.05）。结论：制何首乌水提物20 g/kg对大鼠肝CYP1A2酶活性和mRNA的表达有明显抑制作用。

English Summary:

To observe the effects of water extraction of processed Polygonum multiflorum on enzymatic activities and mRNA expressions of CYP1A2 in rats. Methods:The water extraction of processed Polygonum multiflorum was obtained through extraction with water and vacuum drying and the main components were detected by HPLC. Rats were randomly divided into the blank control group and 2 g/kg BW and 20 g/kg BW the water extraction of processed Polygonum multiflorum group. The administration group was continuously given with water extraction of processed Polygonum multiflorum 10 mL/kg a day, and the blank control group was given the same volume distilled water. After successive administration of 7 d, the liver microsomes were prepared from isolated rat liver, and metabolism elimination rates of phenacetin of CYP1A2 enzyme substrate were assayed by HPLC to determine CYP1A2 enzyme activities in rat liver microsomes of each group. Meanwhile, total RNA of rat liver were extracted to determine the mRNA expressions of CYP1A2 in rat liver by qRT-PCR. Results:Compared with those in blank control group, the enzymatic activities and the mRNA expressions of CYP1A2 in 20 g/kg water extraction of processed Polygonum multiflorum treated group were significantly decreased(enzymatic activities of CYP1A2, P<0.01; mRNA expressions of CYP1A2, P<0.05). Conclusion:20 g/kg water extraction of processed Polygonum multiflorum has significant inhibitory effect on the enzymatic activities and the mRNA expressions of CYP1A2 in rat’s liver microsomes.

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