| 引用本文:年宏蕾，刘仁慧，肖般若，惠娅，杨燕，罗良涛，许利平，王秀娟.淫羊藿女贞子影响激素干预哮喘大鼠淋巴细胞凋亡的作用[J].世界中医药,2016,(03):. |
|
| 淫羊藿女贞子影响激素干预哮喘大鼠淋巴细胞凋亡的作用 |
| Effects of the Combination of Herba Epimedii and Fructus Ligustri Lucidi on Lymphocyte Apoptosis in the Asthmatic Rats with Glucocorticoid Intervention |
| 投稿时间：2015-07-20 |
| DOI：10.3969/j.issn.1673-7202.2016.03.030 |
| 中文关键词: 哮喘 淫羊藿/女贞子 糖皮质激素 淋巴细胞凋亡 大鼠 |
| English Keywords:Asthma Herba Epimedii/ Fructus Ligustri Lucidi Glucocorticoid Lymphocyte apoptosis Rat |
| 基金项目:国家自然科学基金资助项目（编号：81373814）；北京市属高等学校高层次人才引进与培养计划项目（编号：CIT&TCD201504097）；北京中医药薪火传承“3+3”工程二室一站建设项目（编号：2012-SZ-C-42） |
|
| 摘要点击次数: 1333 |
| 全文下载次数: 1401 |
| 中文摘要: |
| 目的：研究大鼠哮喘模型激素局部及全身给药治疗的同时合用淫羊藿女贞子对淋巴细胞凋亡的影响。方法：建立大鼠哮喘模型，分别以布地奈德雾化4周（局部给药）及地塞米松腹腔注射治疗2周（全身给药）治疗，同时配合灌胃淫羊藿女贞子煎液，流式细胞术检测支气管肺泡灌洗液（BALF）与血淋巴细胞凋亡的表达。结果：与正常组相比，哮喘大鼠BALF中淋巴细胞凋亡显著降低（P<0.05）；激素局部干预及合用中药淫羊藿女贞子均可上调BALF中淋巴细胞凋亡水平（P<0.01），且合用组上调作用较单用组显著升高（P<0.01）；而激素全身干预大鼠血及BALF中淋巴细胞凋亡表达均显著上调（P<0.05），但合用淫羊藿女贞子后则下调血淋巴细胞凋亡水平接近于正常（P<0.05）。结论：激素局部给药的同时合用平补阴阳药对淫羊藿女贞子，产生协同上调血及BALF淋巴细胞凋亡的作用；而激素全身给药的同时合用平补阴阳药对淫羊藿女贞子，则抑制激素全身应用后过度促进血淋巴细胞凋亡的影响。 |
| English Summary: |
| To investigate the effects of the combination of Herba Epimedii and Fructus Ligustri Lucidi on lymphocyte apoptosis in the asthmatic rats with glucocorticoid intervention. Methods: Asthmatic model was duplicated and the lymphocyte apoptosis in the blood and BALF was observed by flow cytometry with the oral administration of the combination of Herba Epimedii and Fructus Ligustri Lucidi accompanied by the injection of dexamethasone for two weeks (systemic administration) and the atomization inhalation of Budesonide for four weeks (local administration). Results: Compared with the normal group, apoptosis of lymphocytes in BALF of the asthmatic rats decreased significantly (P<0.05). Both the Budesonide group and the combination group of Budesonide, Herba Epimedii and Fructus Ligustri Lucidi could increase the lymphocyte apoptosis of BALF (P<0.01). The lymphocyte apoptosis of the blood and BALF in the dexamethasone group was significantly increased (P<0.05), while reduced in the combination group of dexamethasone, Herba Epimedii and Fructus Ligustri Lucidi (P<0.05). Conclusion: The local administration of glucocorticoid combined with Herba Epimedii and Fructus Ligustri Lucidi could produce a synergistic upregulation effect on lymphocyte apoptosis, while the systemic administration of glucocorticoid combined Herba Epimedii and Fructus Ligustri Lucidi could inhibit the over-expression of lymphocyte apoptosis. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
