| 引用本文:李秀荣1，李慧杰1，许艳艳2.纳米雄黄抑制人乳腺癌MCF-7细胞上皮间质转化的机制探讨[J].世界中医药,2016,(03):. |
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| 纳米雄黄抑制人乳腺癌MCF-7细胞上皮间质转化的机制探讨 |
| The Mechanism of Nanometer Realgar to Inhibit the Epithelial Mesenchymal Transition of Breast Cancer MCF-7 cells |
| 投稿时间：2015-07-01 |
| DOI：10.3969/j.issn.1673-7202.2016.03.033 |
| 中文关键词: 纳米雄黄 乳腺癌 上皮间质转化 肿瘤转移 |
| English Keywords:Nanometer realgar Breast cancer EMT Metastasis |
| 基金项目:山东省自然科学基金资助项目(编号:ZR2010HL050) |
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| 中文摘要: |
| 目的:观察纳米雄黄对乳腺癌MCF-7细胞上皮间质转化的影响,并探讨其相关机制。方法:体外培养乳腺癌MCF-7细胞,不同浓度纳米雄黄溶液干预,并设立对照组,免疫组化实验检测各组细胞E-cad、HIF-1α表达情况,RT-PCR检测SnailmRNA表达情况。结果:各纳米雄黄组E-cad表达阳性率均明显高于对照组(P<0.01),而HIF-1α表达阳性率均低于对照组(P<0.01);对照组、纳米雄黄低、中、高剂量组、阿霉素组的SnailmRNA相对表达量依次为(52.16±3.87)、(30.10±2.48)、(47.08±3.45)、(41.42±4.35)、(36.07±2.45)。结论:纳米雄黄可通过上调E-cad表达、下调HIF-1α及Snail mRNA表达逆转乳腺癌MCF-7细胞上皮间质转化发挥抗肿瘤转移作用。 |
| English Summary: |
| To explore the effect of nanometer realgar affecting epithelial mesenchymal transition of the breast cancer MCF-7 cells and its possible mechanism. Methods:Breast cancer MCF-7 cells were cultured in vitroand and use different doses of nanometer realgar to intervene. A control group was made. To test the expression of E-cad and HIF-1α, RT-PCR to detected the expression of Snail mRNA. Results:Compared with the control group,the E-cad positive rates of the nanometer realgar group were higher than the control group(P<0.01); the HIF-1α positive rates of the nanometer realgar group were less than the control group(P<0.01);the control group, nanometer realgar low, medium and high dose groups, doxorubicin group of Snail mRNA relative expression levels were （52.16±3.87）, （30.10±2.48）, （47.08±3.45）, （41.42±4.35）, （36.07±2.45） respectively. Conclusion:Nanometer realgar can inhibit the migration and invasion of breast cancer MCF-7 cells, and it has anti-tumor metastasis. Nanometer realgar can resist tumor through reversing EMT by raising the level of E-cad and reducing the level of HIF-1α and Snail mRNA. |
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