| 引用本文:余文珍1，陈实2，张永亮2，涂春香1，陈勇1.活血化瘀法对鼠颈动脉粥样硬化损伤血管wnt/β-catenin信号通路的影响[J].世界中医药,2016,(03):. |
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| 活血化瘀法对鼠颈动脉粥样硬化损伤血管wnt/β-catenin信号通路的影响 |
| Effect of Activating Blood and Resolving Stasis Therapy on Injuried Vascular wnt/β-catenin Signal Pathway of Rats with Carotid Atherosclerosis |
| 投稿时间：2015-07-10 |
| DOI：10.3969/j.issn.1673-7202.2016.03.034 |
| 中文关键词: 活血化瘀法 颈动脉粥样硬化 血管损伤 wnt/β-catenin信号通路 |
| English Keywords:Activating blood and resolving stasis therapy Carotid atherosclerosis Injuried vascular wnt/β-catenin signal pathway |
| 基金项目:福建省教育厅A类科技项目(编号:JA13165);福州市科技计划项目(编号:2012-S-155-1);福建中医药大学校管科研项目(编号:X2012018) |
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| 中文摘要: |
| 目的:观察活血化瘀法对颈动脉粥样硬化血管内皮损伤模型大鼠wnt/β-catenin信号通路的影响,探讨其可能的作用机制。方法:32只SD大鼠高脂喂养1个月后,随机取8只作为假手术组(仅切开皮肤),其余大鼠均行颈总动脉球囊损伤术,成膜后再随机分为模型组(n=8只)、活血化瘀中药方中剂量(临床等效量)组(n=8只)和高剂量(临床2倍量)组(n=8只)。从术后第4天开始予以相应药物灌胃治疗(模型组、假手术组灌等量生理盐水),2周后取术侧颈总动脉段,采用实时荧光定量PCR法捡测wnt、FzD1、AXIN1、GSK-3β、β-catenin、VEGF mRNA基因表达水平。结果:与假手术组比较,模型组wnt、FzD1、AXIN1、β-catenin、VEGF等mRNA水平降低,差异有统计学意义(P<0.01),经活血化瘀中药方干预后,上述各基因mRNA水平均有不同程度升高,尤其是高剂量组与模型组比较差异有统计学意义(P<0.05~0.01);只有GSK-3βmRNA水平各组无明显差异。结论:活血化瘀法可通过上调wnt/β-catenin信号通路表达,使损伤血管VEGFmRNA基因表达水平上升,提示该法可保护颈动脉粥样硬化血管内皮损伤。 |
| English Summary: |
| To observe the effect of activating blood and resolving stasis on injuried vascular wnt/β-catenin in carotid atherosclerosis mode rats and its possible mechanism. Methods:After one-month high fat-diet, 32 SD rats were randomly divided into sham operation group, model group, middle dose (dose in clinical equivalent) group and high dose of (2 times the amount of clinical) group. Except for the sham operation group, the other rats underwent carotid artery balloon injury surgery. After 3 days of intraperitoneally administered orally, model group and sham operation group were given equal volume of physiological saline, administered continuously for 14d. Then the expression of wnt, FzD1, AXIN1, GSK-3β, β-catenin, VEGF mRNA in injured vascular were analyzed by real-time fluorescence quantitative PCR in all groups. Results:Compared with the sham operation group, the expression of Wnt, FzD1, AXIN1, β-catenin, and VEGF mRNA levels in model group decreased(P<0.01); and after treated by activing blood and resolving stasis therapy, the expression of the above genes increased in different degrees, especially in the high dose group (P<0.01~0.05); but there was no significant difference among groups on the expression of GSK-3β mRNA. Conclusion:The theapy of activating blood and resloving stasis therapy can increase the expression of VEGF mRNA of balloon injured carotid artery vascular through up-regulating wnt/β-catenin signal pathway, which suggested that it can protect the injured vascular of carotid atherosclerosis. |
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