| 引用本文:崔俊波1,2，指导:陈宝贵2.回神颗粒减轻中度创伤性脑损伤大鼠脑内炎性反应的机制[J].世界中医药,2016,(03):. |
|
| 回神颗粒减轻中度创伤性脑损伤大鼠脑内炎性反应的机制 |
| The Mechanism of Huishen Granule Alleviating Intracerebral Inflammatory Response on Moderate Traumatic Brain Rats |
| 投稿时间：2015-06-30 |
| DOI：10.3969/j.issn.1673-7202.2016.03.036 |
| 中文关键词: 创伤性脑损伤 继发性脑损伤 脑内炎性反应 中性粒细胞 细胞间黏附分子-1 回神颗粒 |
| English Keywords:Traumatic brain injury Secondary brain injury Intracerebral inflammatory response Polymorphonuclear leukocyte ICAM-1 Huishengranule |
| 基金项目:基金项目:全国名老中医传承工作室建设项目［编号:国中医药人教发(2011)41］;全国中医药传承博士后资助项目［编号:国中医药人教函(2013)240号］;天津市“131”创新型人才第一层次人选培养工程［编号:津人才(2013)9］ |
|
| 摘要点击次数: 1257 |
| 全文下载次数: 1362 |
| 中文摘要: |
| 目的:探讨回神颗粒减轻中度创伤性脑损伤(Traumatic Brain Injury，TBI)后脑内炎性反应的作用环节。方法:应用液压性脑损伤装置建立中度TBI模型,创伤压力为(170±10)kPa,作用时间为(20±2)ms,随机将大鼠分为6组:正常组、假手术组、模型3 d组、模型7 d组、治疗3 d组、治疗7 d组。治疗3 d组和治疗7 d组于创伤后即刻用2 mL药液(含回神颗粒0.27 g)灌胃1次,此后再分别于每日早晚各灌胃1次,每次2 mL药液。采用RT-PCR检测6组动物损伤区脑组织内ICAM-1的变化。结果:与正常组比较:模型3 d组、模型7 d组、治疗3 d组ICAM-1的表达明显升高,具有统计学意义(P<0.01);治疗7 d组ICAM-1的表达的升高无统计学意义(P>0.05)。治疗3 d组ICAM-1的表达较模型3 d组明显降低,差异具有统计学意义(P<0.05);治疗7 d组ICAM-1的表达与模型7 d组比较,差异具有统计学意义(P<0.05)。结论:回神颗粒通过从转录水平抑制ICAM-1的表达,进而减轻脑内炎性反应,避免TBI后脑损伤的进一步加重。 |
| English Summary: |
| This research aims to investigate the mechanism of Huishen Granule alleviating intracerebral inflammatory response in rats with traumatic injury injury(TBI). Methods:Using the equipment of fluid percussion injury (FPI) to establish moderate FPI model in SD rats.The traumatic pressure was (170±10)kPa, traumatic time was (20±2)ms. Randomly divided rats into 6 groups: normal group, sham group, model 3 day group, model 7 day group, experimental 3 day group, experimental 7 day group. Experimental 3 day group and experimental 7 day group were given 2 mL fluid(contain 0.27 g Huishen Granule) into rats’ stomach instantly after trauma, and then given 2 mL fluid in the morning and at night. Using real-time polymerization chain reaction(RT-PCR) to examine the change of ICAM-1 in all 6 groups. Results:Compared with normal group, the ICAM-1 of model 3 day group, model 7 day group and experimental 3 day group increased obviously(P<0.01). However, compared with model 3 day group, the ICAM-1 of experimental 3 day group descended significantly(P<0.05). Compared with model 7 day group, the ICAM-1 of experimental 7 day group declined obviously(P<0.05). Conclusion:The mechanism of Huishen Granule alleviating intracerebral inflammatory response was through prohibiting the expression of ICAM-1 and avoid aggravation of TBI. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
