| 引用本文:邓奕辉，覃弘宇，文果，刘文华，李定祥.化瘀解毒法对MCAO大鼠凝血酶及其受体、脑组织MCP-1、NF-κB的表达及中性粒细胞浸润的影响[J].世界中医药,2016,(04):. |
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| 化瘀解毒法对MCAO大鼠凝血酶及其受体、脑组织MCP-1、NF-κB的表达及中性粒细胞浸润的影响 |
| Effects of Stasis-resolving and Detoxificating Method on Expression of Thrombin and its Receptor, Monocyte Chemoat-tractant Protein (MCP-1), Nuclear Factor κB (NF-κB), and Infiltration of Neutrophil in MCAO Rats |
| 投稿时间：2016-03-24 |
| DOI：10.3969/j.issn.1673-7202.2016.04.003 |
| 中文关键词: 急性缺血性中风 化瘀解毒法 凝血酶 MCP-1 NF-κB 中性粒细胞浸润 |
| English Keywords:Acute ischemic stroke Stasis-resolving and detoxificating method MCP-1 NF-κB Infiltration of neutrophil |
| 基金项目:湖南省自然科学基金项目（编号：14JJ2113）；湖南省中医药科研计划项目（编号：201319；201606）；湖南省教育厅资助项目（编号：12A105） |
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| 中文摘要: |
| 目的：观察化瘀解毒法对MCAO大鼠凝血酶及其受体、大鼠脑组织中单核细胞趋化蛋白（MCP-1）、核因子κB（NF-κB）的表达及中性粒细胞浸润的影响,探讨凝血酶与炎性因子之间的关联性及化瘀解毒法的干预作用。方法：MCAO大鼠分手术组、模型组、活血化瘀法组、清热解毒法组、化瘀解毒法组、阿加曲班组、PDTC组等7组，分组给药，检测各组大鼠给药后脑组织凝血酶及其受体、MCP-1和NF-κB的mRNA表达和蛋白表达水平，并观察缺血区脑组织中性粒细胞浸润情况。结果：模型组脑组织凝血酶及其受体、MCP-1、NF-κB的mRNA表达和蛋白表达均增强（P<0.05），各治疗组治疗后上述指标均下降（P<0.05），化瘀解毒法组较明显；各组大鼠脑组织内PAR-1、MCP-1、NF-κB的蛋白含量与凝血酶蛋白含量均呈正向直线相关（P<0.05）；模型大鼠缺血脑组织中性粒细胞浸润明显，各治疗组均有明显改善。结论：凝血酶毒性和相关炎症反应之间存在关联性，化瘀解毒法可以抑制MCAO大鼠凝血酶毒性和炎症反应，活血化瘀法和清热解毒法之间存在协同作用。 |
| English Summary: |
| To observe the effects of stasis-resolving and detoxificating method on the expression of thrombin and its receptor (PAR-1), monocyte chemoattractant protein (MCP-1) , nuclear factor κB (NF-κB) and infiltration of neutrophil in brain tissue of MCAO rats, and to discuss the association between thrombin and inflammatory factors and the intervention effects of stasis-resolving and detoxificating method.Methods:The MCAO rats were randomly divided into 7 groups: the sham-operated group, the model group, the blood-activating and stasis-resolving method group, the heat-clearing and detoxificating method group, the stasis-resolving and detoxificating method group, the Argatroban group and the PDTC group. And were given the drugs respectively, then the levels of thrombin and its receptor, MCP-1, NF-κB were detected after being given drugs in brain tissue of MCAO rats, and the infiltration of neutrophil in ischemic area was observed.Results:The mRNA and protein expressions of thrombin and its receptor, MCP-1, NF-κB in brain tissue of the model group increased obviously (P<0.05), while all of those decreased (P<0.05) in each treated groups after treatment, and it also present a markedly reduction in the stasis-resolving and detoxificating group. A positive linear correlation was shown in contents of protein of PAR-1, MCP-1, NF-κB and thrombin in brain tissue of each group (P<0.05). The infiltration of neutrophil in ischemic brain tissue of the model rats was visible, and there were obvious improvements in each treated groups.Conclusion:It is found that there was a correlation between thrombin toxicity and inflammation responses, and both of them were inhibited by stasis-resolving and detoxificating in MCAO rats. Furthermore, there was an obvious synergistic effect in stasis-resolving and detoxificating and heat-clearing and detoxicating method group. |
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